RRC ID 31282
Author Yabuki A, Nakayama T, Yubuki N, Hashimoto T, Ishida K, Inagaki Y.
Title Tsukubamonas globosa n. gen., n. sp., a novel excavate flagellate possibly holding a key for the early evolution in "Discoba".
Journal J. Eukaryot. Microbiol.
Abstract We report the ultrastructure and phylogenetic position of a free-living heterotrophic flagellate, Tsukubamonas globosa n. gen., n. sp. This flagellate was isolated from a pond in the University of Tsukuba, Japan. Under light microscopy, the spherical vegetative cells were naked and highly vacuolated, and always swam with rotating motion. Electron microscopic observations revealed that T. globosa possessed a ventral feeding groove, which is one of the hallmark characteristics of the supergroup Excavata. The position of T. globosa was unresolved in the small subunit ribosomal RNA phylogeny. On the other hand, a multigene phylogeny using α-tubulin, β-tubulin, actin, heat shock protein 90, and translation elongation factor 2 robustly united T. globosa with members of the "Discoba" clade of Excavata, composed of jakobids, euglenozoans, and heteroloboseans, although the precise position of T. globosa in this clade remained unresolved. Our detailed morphological comparisons elucidated that T. globosa possessed a novel set of morphological features, and could not be classified into any taxa in the Discoba clade. Instead we classified T. globosa into Tsukubamonadidae n. fam. under Tsukubamonadida n. ord.
Volume 58(4)
Pages 319-31
Published 2011-7
DOI 10.1111/j.1550-7408.2011.00552.x
PMID 21569159
MeSH Actins / genetics Aquatic Organisms / classification Aquatic Organisms / genetics Aquatic Organisms / isolation & purification Biological Evolution* Cytoskeleton / ultrastructure DNA, Ribosomal / chemistry DNA, Ribosomal / classification DNA, Ribosomal / genetics* Euglenozoa / classification Euglenozoa / genetics Eukaryota / classification* Eukaryota / genetics Eukaryota / isolation & purification* Eukaryota / ultrastructure Flagella Freshwater Biology Heat-Shock Proteins / genetics Japan Microscopy, Electron Molecular Sequence Data Phylogeny Polymerase Chain Reaction Ponds RNA, Ribosomal / genetics Sequence Alignment Tubulin / genetics
IF 2.537
Times Cited 12
WOS Category MICROBIOLOGY
Resource
Algae NIES-1390