RRC ID 31327
Author Hoef-Emden K.
Title Osmotolerance in the Cryptophyceae: jacks-of-all-trades in the Chroomonas Clade.
Journal Protist
Abstract No detailed studies have been performed to date on osmotolerance in cryptophytes, although one species, Chroomonas africana, had previously been reported to grow in freshwater as well as seawater. This study focused on osmotolerance in Chroomonas. Growth at different osmolalities and parameters of contractile vacuole function were examined and compared across a high-resolution phylogeny. Two evolutionary lineages in the Chroomonas clade proved to be euryhaline. Ranges of osmotolerance depended not only on osmolality, but also on culture medium. All cryptophytes contained contractile vacuoles. In the euryhaline strain CCAP 978/08 contractile vacuoles could be observed even at an osmolality beyond that of seawater. In addition the cells accumulated floridoside, an osmoprotectant likely originating from the red algal carbohydrate metabolism of the complex rhodoplast. Further evidence for functional contractile vacuoles also in marine cryptophytes was provided by identification of contractile vacuole-specific genes in the genome of Guillardia theta.
Volume 165(2)
Pages 123-43
Published 2014-3
DOI 10.1016/j.protis.2014.01.001
PII S1434-4610(14)00002-9
PMID 24568876
MeSH Cluster Analysis Cryptophyta / classification Cryptophyta / genetics Cryptophyta / physiology* Culture Media / chemistry DNA, Algal / chemistry DNA, Algal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fresh Water Genes, rRNA Molecular Sequence Data Osmotic Pressure* Phylogeny RNA, Ribosomal, 18S / genetics Seawater Sequence Analysis, DNA Sequence Homology, Nucleic Acid Stress, Physiological* Vacuoles / physiology
IF 2.702
Times Cited 3
Algae NIES-712 NIES-713 NIES-714 NIES-708 NIES-1004