Reference - Detail
| RRC ID | 31333 |
|---|---|
| Author | Su Y, Wang J, Shi M, Niu X, Yu X, Gao L, Zhang X, Chen L, Zhang W. |
| Title | Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions. |
| Journal | Bioresour Technol |
| Abstract |
Various combinations of acetate (Ac), Fe(2+) and high light (HL) stress conditions were evaluated to maximize astaxanthin accumulation and biomass production in Haematococcus pluvialis, and then GC-MS and LC-MS based metabolomics were applied to determine molecular mechanisms responsible for enhancing astaxanthin accumulation under the stress conditions. With the optimized analytical protocols, the GC-MS and LC-MS analyses allowed identification of 93 stable and 24 unstable intracellular metabolites from H. pluvialis, respectively. In addition, a metabolic network was constructed based on GC-MS metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 2, 1 and 1 distinguished metabolic modules highly associated with HL, Fe(2+) & HL, and Ac & Fe(2+) & HL conditions, respectively. Finally, LC-MS analysis found that AKG, Glu and R5P may be metabolites associated with the Fe(2+) & HL condition. The study provided the first metabolomic view of cell growth and astaxanthin accumulation in H. pluvialis. |
| Volume | 170 |
| Pages | 522-529 |
| Published | 2014-10-1 |
| DOI | 10.1016/j.biortech.2014.08.018 |
| PII | S0960-8524(14)01125-0 |
| PMID | 25164345 |
| MeSH | Chlorophyta / metabolism Chlorophyta / physiology* Chromatography, Liquid / methods Gas Chromatography-Mass Spectrometry / methods Metabolic Networks and Pathways / genetics Metabolic Networks and Pathways / physiology* Metabolome / genetics Metabolome / physiology* Metabolomics / methods* Stress, Physiological / physiology* Xanthophylls / biosynthesis Xanthophylls / genetics Xanthophylls / metabolism |
| IF | 7.539 |
| Times Cited | 46 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY AGRICULTURAL ENGINEERING ENERGY & FUELS |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-144 |