論文 - 詳細
| RRC ID | 31339 |
|---|---|
| 著者 | Kato A, Fujii E, Watanabe T, Takashima Y, Matsushita H, Furuhashi T, Morita A. |
| タイトル | Distribution of IL-31 and its receptor expressing cells in skin of atopic dermatitis. |
| ジャーナル | J Dermatol Sci |
| Abstract |
BACKGROUND:To understand the clinical segments of IL-31 signaling blockade therapy in pruritus of atopic dermatitis (AD), direct detection of the target proteins in the diseased tissues will provide crucial information. There is a lack of direct evidence concerning the cellular origin of IL-31 in AD skins, and data on the expression of IL-31RA in AD are inconsistent. Also, there is no available information regarding IL-31RA protein expression in human dorsal root ganglia (DRG), which mediates the sensation of itch and is the long-suspected source of the protein. OBJECTIVE:We sought to obtain direct evidence concerning the distribution of IL-31- and IL-31RA-protein expressing cells and their characteristics in AD skin samples and in human DRG. METHODS:IL-31 was detected immunohistochemically in AD skins, and representative sections were double stained with IL-31 and several immune-markers. IL-31RA was stained immunohistochemically in AD skins and normal human DRG, and representative AD skins were double stained with IL-31RA and PGP9.5 (a nerve marker). RESULTS:IL-31-positive cells were observed as mononuclear infiltrating cells and as CD11b co-expressing cells in severe AD samples. As for IL-31RA, positive reactions were detected in keratinocytes and nerve fibers in the dermis of AD and in the neurons of normal DRG. CONCLUSION:The detection of IL-31 in infiltrating cells of severe AD skin and of IL-31RA in nerve fibers of AD dermis and normal DRG indicates IL-31 signaling may be a contributing factor in the persistence and exacerbation of AD skin lesions. |
| 巻・号 | 74(3) |
| ページ | 229-35 |
| 公開日 | 2014-6-1 |
| DOI | 10.1016/j.jdermsci.2014.02.009 |
| PII | S0923-1811(14)00059-0 |
| PMID | 24667097 |
| MeSH | Dermatitis, Atopic / metabolism* Humans Interleukins / metabolism* Receptors, Interleukin / metabolism* Skin / metabolism |
| IF | 3.681 |
| 引用数 | 49 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) |