RRC ID 31363
Author Ohnishi Y, Hoshino R, Okamoto T.
Title Dynamics of Male and Female Chromatin during Karyogamy in Rice Zygotes.
Journal Plant Physiol
Abstract In angiosperms, the conversion of an egg cell into a zygote involves two sequential gametic processes: plasmogamy, the fusion of the plasma membranes of male and female gametes, and karyogamy, the fusion of the gametic nuclei. In this study, the nuclei and nuclear membranes of rice (Oryza sativa) gametes were fluorescently labeled using histones 2B-green fluorescent protein/red fluorescent protein and Sad1/UNC-84-domain protein2-green fluorescent protein, respectively, which were heterologously expressed. These gametes were fused in vitro to produce zygotes, and the nuclei and nuclear membranes in the zygotes were observed during karyogamy. The results indicated that the sperm nucleus migrates adjacent to the egg nucleus 5 to 10 min after plasmogamy via an actin cytoskelton, and the egg chromatin then appears to move unidirectionally into the sperm nucleus through a possible nuclear connection. The enlargement of the sperm nucleus accompanies this possible chromatin remodeling. Then, 30 to 70 min after fusion, the sperm chromatin begins to decondense with the completion of karyogamy. Based on these observations, the development of early rice zygotes from plasmogamy to karyogamy was divided into eight stages, and using reverse transcription PCR analyses, paternal and de novo synthesized transcripts were separately detected in zygotes at early and late karyogamy stages, respectively.
Volume 165(4)
Pages 1533-1543
Published 2014-8-1
DOI 10.1104/pp.114.236059
PII pp.114.236059
PMID 24948834
PMC PMC4119036
IF 6.305
Times Cited 32
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes rpc00031