| RRC ID |
3139
|
| 著者 |
Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa J.
|
| タイトル |
Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors.
|
| ジャーナル |
Jpn J Infect Dis
|
| Abstract |
We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
|
| 巻・号 |
60(1)
|
| ページ |
45-7
|
| 公開日 |
2007-2-1
|
| PMID |
17314425
|
| MeSH |
Cloning, Molecular
Drug Resistance, Bacterial
Escherichia coli / genetics*
Genetic Vectors / genetics*
Lac Operon
Mycobacterium / genetics
Nocardia / genetics*
Plasmids / genetics
Tobramycin / pharmacology
Transformation, Bacterial / genetics
|
| IF |
1.24
|
| 引用数 |
38
|
|
WOS 分野
|
INFECTIOUS DISEASES
|
| リソース情報 |
| 病原微生物 |
N. farcinica(IFM 10152)
N. asteroides(IFM 0319)
E. coli |
