RRC ID 3143
Author Hasegawa T, Gonoi T, Ito J, Kogure T, Yazawa K, Mikami Y.
Title Identification of Nocardia farcinica by a PCR primer amplifying a specific DNA band for the bacterium.
Journal Nihon Ishinkin Gakkai Zasshi
Abstract A PCR primer specific to Nocardia farcinica was prepared based on sequence information of random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that are frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL).
Volume 48(4)
Pages 173-5
Published 2007-1-1
DOI 10.3314/jjmm.48.173
PMID 17975533
MeSH Animals Blood / microbiology Bronchoalveolar Lavage Fluid / microbiology DNA Primers* DNA, Bacterial / analysis* Humans Mice Nocardia / genetics* Nocardia / isolation & purification* Nocardia Infections / diagnosis* Nocardia Infections / microbiology* Random Amplified Polymorphic DNA Technique / methods* Sputum / microbiology
Pathogenic microorganisms IFM 0236 IFM 0239 IFM 0284 IFM 0290 IFM 0319 IFM 0333 IFM 10029 IFM 10031 IFM 10064 IFM 10084 IFM 10152 IFM 10580 IFM 10821 IFM 10823 IFM 10826 IFM 10835 IFM 10836 IFM 10840 IFM 10853