| RRC ID |
3144
|
| 著者 |
Higurashi H, Arai M, Watanabe A, Igari H, Seki N, Kamei K, Kuriyama T.
|
| タイトル |
Gene expression profiling of polymorphonuclear leukocytes treated with the culture filtrate of Aspergillus fumigatus and gliotoxin.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
Pathogens of the Aspergillus species are frequently seen in deep-seated mycoses. We previously demonstrated that the culture filtrate of Aspergillus fumigatus (CF) has immunosuppressive effects on polymorphonuclear leukocytes (PMNs), which act as the main phagocytes to hyphae of Aspergillus fumigatus (A. fumigatus). But little is known about the gene expression profiles involved in it. Therefore we investigated the changes in gene expression in human PMNs treated with CF or gliotoxin at two time points, using microarray analysis. CF and gliotoxin changed the expression of 548 and 381 genes, respectively. Only 51 genes showed the same expression patterns with the two stimulants, and CF-induced changes in gene expression occurred comparatively earlier than those induced by gliotoxin. Among 31 genes encoding apoptosis, which were up- or down-regulated in this assay, only 3 genes were similarly changed by both kinds of stimulation. Apoptosis was detected and quantified using two apoptosis assays. CF and gliotoxin changed the expessions of only 3 out of 19 regulated genes related to inflammatory mediators and receptors similarly. The up-regulation of the gene encoding annexin 1 (ANXA1), which is known to be involved in extravasation and apoptosis of neutrophils, may play a role in the immunosuppressive effect of A. fumigatus. The difference in expression changes between CF and gliotoxin is presumed to be caused by the interaction among the components of CF and therefore the interaction is an area of interest for further investigation.
|
| 巻・号 |
51(4)
|
| ページ |
407-19
|
| 公開日 |
2007-1-1
|
| DOI |
10.1111/j.1348-0421.2007.tb03928.x
|
| PII |
JST.JSTAGE/mandi/51.407
|
| PMID |
17446680
|
| MeSH |
Apoptosis / physiology
Aspergillus fumigatus / metabolism*
Gene Expression Profiling*
Gene Expression Regulation, Fungal
Genes, Fungal
Gliotoxin / analysis
Gliotoxin / biosynthesis
Gliotoxin / pharmacology*
Neutrophils / drug effects*
Neutrophils / metabolism
|
| IF |
1.566
|
| 引用数 |
8
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 47450
IFM 47042? |
