| RRC ID |
3149
|
| Author |
Pandey K, Yanagi T, Pandey BD, Mallik AK, Sherchand JB, Kanbara H.
|
| Title |
Characterization of Leishmania isolates from Nepalese patients with visceral leishmaniasis.
|
| Journal |
Parasitol Res
|
| Abstract |
In Nepal, visceral leishmaniasis (VL) is endemic in 13 districts of the central and eastern regions. A total of 166 bone-marrow aspirates were obtained from patients with suspected VL. Ninety-seven were identified as positive by microscopy, and 29 of those were successfully isolated and cultured. We characterized these isolates by molecular analysis and by their ability to infect mice. PCR-restriction fragment length polymorphism analysis of the mini-exon and the cysteine proteinase b gene showed that all isolates were Leishmania donovani, and the restriction pattern of the Nepalese isolates corresponded to the standard Indian strain of L. donovani but differed from that of the Kenyan strain. The single-strand conformation polymorphism analysis of ribosomal internal transcribed spacer showed no genetic heterogeneity within Nepalese isolates. Intraperitoneal inoculation with the promastigotes of all isolates resulted in amastigote proliferation in the spleen of 20 nude mice, of which ten isolates were highly infective, and ten were moderately infective, including one BALB/c mouse. Of the 20 amastigotes isolated from the spleen of nude mice, only the ten highly infective isolates infected BALB/c mice, of which, two isolates were considered to have low infectivity, three isolates were considered to be moderately infective, and five isolates were considered to be highly infective.
|
| Volume |
100(6)
|
| Pages |
1361-9
|
| Published |
2007-5-1
|
| DOI |
10.1007/s00436-007-0464-4
|
| PMID |
17310397
|
| MeSH |
Adult
Aged
Animals
Child
Child, Preschool
Cricetinae
DNA, Protozoan / genetics
DNA, Ribosomal Spacer / genetics
Female
Humans
Leishmania / classification*
Leishmania / genetics*
Leishmania / isolation & purification
Leishmaniasis, Visceral / epidemiology
Leishmaniasis, Visceral / parasitology*
Male
Mesocricetus
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Nepal / epidemiology
|
| IF |
1.641
|
| Times Cited |
18
|
|
WOS Category
|
PARASITOLOGY
|
| Resource |
| Pathogenic microorganisms |
L. donovani(D5
D7
D10
D14
D19
D21
D26
D29
D34
D36
D38
D42
D45
D67?
D70?
D71?
D77?
D78?
D98?
D99?
T4?
T6
T9
T11
T13?
T14?
T22?
T25?
T27?) |
