Reference - Detail
| RRC ID | 3153 |
|---|---|
| Author | Masue N, Deguchi T, Yokoi S, Yamada T, Ohkusu K, Ezaki T. |
| Title | System for simultaneous detection of 16 pathogens related to urethritis to diagnose mixed infection. |
| Journal | Int J Urol |
| Abstract |
OBJECTIVE:Urethritis is not always caused by a single pathogen, and isolation of more than two pathogens from one patient is not uncommon. We developed a method to simultaneously detect 16 pathogens related to urethritis. METHODS:We designed specific primers used for amplification of urethritis pathogens in our 16-well microplate assay. Sixteen microliters of each reaction mixture containing template DNA was added to each well to amplify 16 pathogens simultaneously. RESULTS:After we evaluated the specificity and sensitivity of this microplate polymerase chain reaction method, we used it to detect pathogens in clinical samples. Of 163 clinical samples, 49.7% (81/163) were positive for specific pathogens, and 6.7% (11/163) showed mixed infection. A specific pathogen was not identified in 43.6% (71/163) of cases. CONCLUSIONS:We developed a 16-well microplate assay with 16 specific primers to identify pathogens associated with urethritis. |
| Volume | 14(1) |
| Pages | 39-42 |
| Published | 2007-1-1 |
| DOI | 10.1111/j.1442-2042.2006.01660.x |
| PII | IJU1660 |
| PMID | 17199858 |
| MeSH | Humans Infections / complications Infections / microbiology Microbiological Techniques / methods Polymerase Chain Reaction Urethritis / microbiology* |
| IF | 2.445 |
| Times Cited | 6 |
| WOS Category | UROLOGY & NEPHROLOGY |
| Resource | |
| Pathogenic microorganisms | |