| RRC ID |
3161
|
| Author |
Nhung PH, Hata H, Ohkusu K, Noda M, Shah MM, Goto K, Ezaki T.
|
| Title |
Use of the novel phylogenetic marker dnaJ and DNA-DNA hybridization to clarify interrelationships within the genus Aeromonas.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
The interrelationships of 27 Aeromonas strains were investigated using dnaJ sequences and DNA-DNA hybridization. dnaJ sequence similarities showed a stronger relationship with DNA-DNA relatedness values than did 16S rRNA gene sequence similarities. Additionally, dnaJ sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16S rRNA gene sequences for the differentiation of strains at the species level. Relationships among Aeromonas species were therefore elucidated on the basis of dnaJ sequences and DNA-DNA reassociation. Strains of Aeromonas encheleia and Aeromonas sp. HG11 were unquestionably grouped in the same genetic species, since they shared 98.7 % dnaJ sequence similarity and 82-85 % genomic relatedness. The phylogenetically close relationships obtained from dnaJ sequence analysis (1.7-3.3 % genetic distance) were corroborated by high DNA-DNA relatedness (73-97 %) to support the previous suggestion that Aeromonas culicicola and Aeromonas allosaccharophila are later heterotypic synonyms of Aeromonas veronii. Our findings will contribute to the clarification of controversial relationships in the genus Aeromonas and also demonstrate that analysis of dnaJ sequences can be a powerful tool for interspecies study of the genus.
|
| Volume |
57(Pt 6)
|
| Pages |
1232-1237
|
| Published |
2007-6-1
|
| DOI |
10.1099/ijs.0.64957-0
|
| PMID |
17551035
|
| MeSH |
Aeromonas / classification*
Aeromonas / genetics*
Bacterial Proteins / genetics*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
HSP40 Heat-Shock Proteins / genetics*
Molecular Sequence Data
Nucleic Acid Hybridization*
Phylogeny
Sequence Analysis, DNA
Sequence Homology, Amino Acid
|
| IF |
2.415
|
| Times Cited |
52
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
A. hydrophila subsp. ranaei?
A. hydrophila subsp. hydrophila?
A. hydrophila subsp.dhakensis?
A. trota?
A. enteropelogenes?
A. sobria?
A. jandaei?
A. ichthiosmia?
A. veronii bv.Sobria?
A. culicicola?
A. veronii bv.Veronii?
A. allosaccharophila?
A. molluscorum?
A. salmonicida subsp.pectinolytica?
A. salmonicida subsp.smithia?
A. salmonicida subsp.salmonicida?
A. salmonicida subsp.masoucida?
A. salmonicida subsp.achromogenes?
A. popoffii?
A. bestiarum?
A. media?. A. caviae?
A. eucrenophila?
A. encheleia?
Aeromonas sp.?
A. simiae?
A. schubertii?
V. cholerae? |
