論文 - 詳細
| RRC ID | 3168 |
|---|---|
| 著者 | Shah MM, Iihara H, Noda M, Song SX, Nhung PH, Ohkusu K, Kawamura Y, Ezaki T. |
| タイトル | dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
In the last few years, many attempts have been made to use conserved gene sequences for identification and for phylogenetic studies of Staphylococcus species. In an effort to identify a more reliable approach, a dnaJ gene sequence-based database was created. In this study, an approximately 883 bp portion of the dnaJ gene sequence from 45 staphylococcal type strains was compared with 16S rRNA and other conserved gene (hsp60, sodA and rpoB) sequences available in public databases. Nucleotide sequence comparisons revealed that the staphylococcal dnaJ gene showed higher discrimination (mean similarity 77.6 %) than the 16S rRNA (mean similarity 97.4 %), rpoB (mean similarity 86 %), hsp60 (mean similarity 82 %) and sodA (mean similarity 81.5 %) genes. Analysis of the dnaJ gene sequence from 20 Staphylococcus isolates representing two clinically important species showed <1 % sequence divergence. Phylogenetic data obtained from the dnaJ gene sequence were in general agreement with those of 16S rRNA gene sequence analysis and DNA-DNA reassociation studies. In conclusion, the dnaJ gene sequence-based assay is an effective alternative to currently used methods, including 16S rRNA gene sequencing, for identification and taxonomical analysis of Staphylococcus species. |
| 巻・号 | 57(Pt 1) |
| ページ | 25-30 |
| 公開日 | 2007-1-1 |
| DOI | 10.1099/ijs.0.64205-0 |
| PMID | 17220435 |
| MeSH | Bacterial Typing Techniques* DNA, Bacterial / analysis HSP40 Heat-Shock Proteins / genetics* Humans Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA / methods* Species Specificity Staphylococcus / classification* Staphylococcus / genetics |
| IF | 2.415 |
| 引用数 | 85 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | S. arlettae(GTC 00383) S. aureus(GTC 00371) S. aureus(GTC 00286) S. auricularis(GTC 00326) S. capitis(GTC 00287) S. capitis(GTC 00727) S. caprae(GTC 00378) S. carnosus(GTC 00384) S. carnosus(GTC 01232) S. chromogenes(GTC 00482)? S. cohnii(GTC 00288) S. cohnii(GTC 00728) S. condimenti(GTC 01233) S. delphini(GTC 00476) S. epidermidis(GTC 00289) S. equorum(GTC 00381) S. felis(GTC 00509) S. fleurettii(GTC 01999) S. gallinarum(GTC 00379) S. haemolyticus(GTC 00290) S. hominis(GTC 00485) S. hominis(GTC 01228) S. hyicus(GTC 00305) S. intermedius(GTC 00266) S. kloosii(GTC 00382) S. lentus(GTC 00267) S. lugdunensis(GTC 00458) S. lutrae(GTC 01248) S. muscae(GTC 00704) S. pasteuri(GTC 00705) S. piscifermentans(GTC 00585)? S. pulvereri(GTC 00722) S. saccharolyticus(GTC 00181) S. saprophyticus(GTC 00843) S. saprophyticus(GTC 00265) S. schleiferi(GTC 00510) S. schleiferi(GTC 00483) S. sciuri(GTC 01227) S. sciuri(GTC 00844) S. sciuri(GTC 00291) S. simulans(GTC 00292) S. succinus(GTC 01256) S. vitulinus(GTC 00729) S. warneri(GTC 00293) S. xylosus(GTC 00294) |