RRC ID 31694
Author Noomhorm N, Chang CJ, Wen CS, Wang JY, Chen JL, Tseng LM, Chen WS, Chiu JH, Shyr YM.
Title In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen.
Journal J Pharmacol Sci
Abstract This study investigated the herb-drug interaction of xanthorrhizol and tamoxifen in human breast cancer cells. Using MCF-7 cell line as an in vitro model, the herb-drug interaction between xanthorrhizol and tamoxifen was measured by MTT assay, luciferase reporter assay, and cell cycle analysis. The effects of xanthorrhizol on growth/autophagy related signaling were determined by immunostaining, western blotting, and real time RT-PCR. Additionally, the in vivo effect of xanthorrhizol and tamoxifen on athymic nude mice implanted with MCF-7 cells was evaluated. When MCF-7 cells were co-treated with tamoxifen and xanthorrhizol, there were no significant changes in terms of cell number, luciferase activity, percentage S-phase cells and LC3-II expression. However, using the MCF-7 implanted nude mice model, it was possible to detect significantly increased tumor volumes, a larger tumor size, and increased protein expression of P38 and P27(Kip1) in the xanthorrhizol + tamoxifen group compared to the tamoxifen-alone group. It can be concluded that while there is no significant herb-drug interaction between xanthorrhizol and tamoxifen in vitro, there is such an interaction in tumor-bearing mice, which provides important information that affects breast cancer treatment translational research.
Volume 125(4)
Pages 375-85
Published 2014-1-1
DOI 10.1254/jphs.14024fp
PMID 25141924
MeSH Animals Breast Neoplasms / drug therapy* Breast Neoplasms / genetics Breast Neoplasms / pathology* Cell Transformation, Neoplastic / drug effects* Cyclin-Dependent Kinase Inhibitor p27 / metabolism Disease Models, Animal Drug Therapy, Combination Female Herb-Drug Interactions Humans Luciferases / metabolism MCF-7 Cells Mice, Inbred BALB C Mice, Nude Microtubule-Associated Proteins / metabolism Neoplasm Transplantation Phenols / administration & dosage* Phenols / adverse effects* Phenols / pharmacology Tamoxifen / administration & dosage* Tamoxifen / adverse effects* Tamoxifen / pharmacology
IF 2.835
Times Cited 6
DNA material c-erbB2 promoter(533)-pGL2-basic (RDB02839) pGL4-phESR1 (RDB07528).