論文 - 詳細
| RRC ID | 31694 |
|---|---|
| 著者 | Noomhorm N, Chang CJ, Wen CS, Wang JY, Chen JL, Tseng LM, Chen WS, Chiu JH, Shyr YM. |
| タイトル | In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen. |
| ジャーナル | J Pharmacol Sci |
| Abstract |
This study investigated the herb-drug interaction of xanthorrhizol and tamoxifen in human breast cancer cells. Using MCF-7 cell line as an in vitro model, the herb-drug interaction between xanthorrhizol and tamoxifen was measured by MTT assay, luciferase reporter assay, and cell cycle analysis. The effects of xanthorrhizol on growth/autophagy related signaling were determined by immunostaining, western blotting, and real time RT-PCR. Additionally, the in vivo effect of xanthorrhizol and tamoxifen on athymic nude mice implanted with MCF-7 cells was evaluated. When MCF-7 cells were co-treated with tamoxifen and xanthorrhizol, there were no significant changes in terms of cell number, luciferase activity, percentage S-phase cells and LC3-II expression. However, using the MCF-7 implanted nude mice model, it was possible to detect significantly increased tumor volumes, a larger tumor size, and increased protein expression of P38 and P27(Kip1) in the xanthorrhizol + tamoxifen group compared to the tamoxifen-alone group. It can be concluded that while there is no significant herb-drug interaction between xanthorrhizol and tamoxifen in vitro, there is such an interaction in tumor-bearing mice, which provides important information that affects breast cancer treatment translational research. |
| 巻・号 | 125(4) |
| ページ | 375-85 |
| 公開日 | 2014-1-1 |
| DOI | 10.1254/jphs.14024fp |
| PII | DN/JST.JSTAGE/jphs/14024FP |
| PMID | 25141924 |
| MeSH | Animals Breast Neoplasms / drug therapy* Breast Neoplasms / genetics Breast Neoplasms / pathology* Cell Transformation, Neoplastic / drug effects* Cyclin-Dependent Kinase Inhibitor p27 / metabolism Disease Models, Animal Drug Therapy, Combination Female Herb-Drug Interactions Humans Luciferases / metabolism MCF-7 Cells Mice, Inbred BALB C Mice, Nude Microtubule-Associated Proteins / metabolism Neoplasm Transplantation Phenols / administration & dosage* Phenols / adverse effects* Phenols / pharmacology Tamoxifen / administration & dosage* Tamoxifen / adverse effects* Tamoxifen / pharmacology |
| IF | 2.835 |
| 引用数 | 6 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | c-erbB2 promoter(533)-pGL2-basic (RDB02839) pGL4-phESR1 (RDB07528). |