| RRC ID |
31695
|
| Author |
Kikuchi Y, Kakeya T, Nakajima O, Sakai A, Ikeda K, Yamaguchi N, Yamazaki T, Tanamoto K, Matsuda H, Sawada J, Takatori K.
|
| Title |
Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein.
|
| Journal |
FEBS J
|
| Abstract |
The human prion protein (PrP) is a glycoprotein with a glycosylphosphatidylinositol (GPI) anchor at its C-terminus. Here we report alternative splicing within exon 2 of the PrP gene (PRNP) in the human glioblastoma cell line T98G. The open reading frame of the alternatively spliced mRNA lacked the GPI anchor signal sequence and encoded a 230 amino acid polypeptide. Its product, GPI-anchorless PrP (GPI(-) PrPSV), was unglycosylated and soluble in non-ionic detergent, and was found in the cytosolic fraction. We also detected low levels of alternatively spliced mRNA in human brain and non-neuronal tissues. When long-term passaged T98G cells were placed in a low-oxygen environment, alternatively spliced mRNA expression increased and expression of normally spliced PrP mRNA decreased. These findings imply that oxygen tension regulates GPI(-) PrPSV expression in T98G cells.
|
| Volume |
275(11)
|
| Pages |
2965-76
|
| Published |
2008-6-1
|
| DOI |
10.1111/j.1742-4658.2008.06452.x
|
| PII |
EJB6452
|
| PMID |
18445040
|
| MeSH |
Alternative Splicing
Amino Acid Sequence
Cell Line, Tumor
Cytosol / metabolism
Exons
Gene Expression Regulation*
Glycoproteins / chemistry
Glycosylphosphatidylinositols / metabolism*
Humans
Hypoxia*
Ions
Molecular Sequence Data
Neurons / metabolism
Prions / chemistry*
Prions / genetics*
Protein Structure, Tertiary
|
| IF |
4.392
|
| Times Cited |
20
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
hPrP 129M with signal sequences (RDB12404)
hPrP 129V with signal sequences (RDB12405)
hPrP129M in pTA2 (RDB06716)
hPrP129V in pTA2 (RDB06717)
hPrPSV129M in pTA2 (RDB06718)
hPrPSV129V in pTA2 (RDB06719). |
