| RRC ID |
31702
|
| Author |
Kioka N, Tsubota J, Kakehi Y, Komano T, Gottesman MM, Pastan I, Ueda K.
|
| Title |
P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
We isolated a full-length MDR1 cDNA from human adrenal where P-glycoprotein is expressed at high level. The deduced amino acid sequence shows two amino acid differences from the sequence of P-glycoprotein obtained from colchicine-selected multidrug resistant cultured cells. The amino acid substitution Gly----Val at codon 185 in P-glycoprotein from colchicine resistant cells occurred during selection of cells in colchicine. As previously reported, cells transfected with the MDR1 cDNA carrying Val185 acquire increased resistance to colchicine compared to other drugs. The other amino acid substitution Ser----Ala at codon 893 probably reflects genetic polymorphism. The MDR1 gene, the major member of the P-glycoprotein gene family expressed in human adrenal, is sufficient to confer multidrug-resistance on culture cells.
|
| Volume |
162(1)
|
| Pages |
224-31
|
| Published |
1989-7-14
|
| DOI |
10.1016/0006-291x(89)91985-2
|
| PII |
0006-291X(89)91985-2
|
| PMID |
2568832
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily B, Member 1
Adrenal Glands / analysis*
Adrenal Glands / physiology
Alanine / genetics
Amino Acid Sequence
Base Sequence
Cell Line
Cloning, Molecular
DNA / isolation & purification*
Drug Resistance
Genetic Vectors
Glycine / genetics*
Humans
Membrane Glycoproteins / genetics*
Membrane Glycoproteins / physiology
Molecular Sequence Data
Polymorphism, Genetic*
Serine / genetics
Tumor Cells, Cultured / analysis
Tumor Cells, Cultured / metabolism
|
| IF |
2.985
|
| Times Cited |
162
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
Human MDR1 cDNA (RDB01372) |
