| RRC ID |
31703
|
| Author |
Chen CJ, Chin JE, Ueda K, Clark DP, Pastan I, Gottesman MM, Roninson IB.
|
| Title |
Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells.
|
| Journal |
Cell
|
| Abstract |
Resistance of tumor cells to multiple cytotoxic drugs is a major impediment to cancer chemotherapy. Multidrug resistance in human cells is determined by the mdr1 gene, encoding a high molecular weight membrane glycoprotein (P-glycoprotein). Complete primary structure of human P-glycoprotein has been determined from the cDNA sequence. The protein, 1280 amino acids long, consists of two homologous parts of approximately equal length. Each half of the protein includes a hydrophobic region with six predicted transmembrane segments and a hydrophilic region. The hydrophilic regions share homology with peripheral membrane components of bacterial active transport systems and include potential nucleotide-binding sites. These results are consistent with a function for P-glycoprotein as an energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
|
| Volume |
47(3)
|
| Pages |
381-9
|
| Published |
1986-11-7
|
| DOI |
10.1016/0092-8674(86)90595-7
|
| PII |
0092-8674(86)90595-7
|
| PMID |
2876781
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily B, Member 1
Amino Acid Sequence
Bacterial Proteins / analysis
Bacterial Proteins / genetics*
Base Sequence
Biological Transport, Active
Cell Line
DNA / analysis
Drug Resistance / genetics*
Glycoproteins / analysis
Glycoproteins / genetics*
Humans
Molecular Weight
Neoplasms / drug therapy
Protein Conformation
|
| IF |
38.637
|
| Times Cited |
1861
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
Human MDR1 cDNA (RDB01372) |
