| RRC ID |
3171
|
| 著者 |
Tachibana H, Yanagi T, Pandey K, Cheng XJ, Kobayashi S, Sherchand JB, Kanbara H.
|
| タイトル |
An Entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from Entamoeba histolytica.
|
| ジャーナル |
Mol Biochem Parasitol
|
| Abstract |
An Entamoeba sp. strain, P19-061405, was isolated from a rhesus monkey in Nepal and characterized genetically. The strain was initially identified as Entamoeba histolytica using PCR amplification of peroxiredoxin genes. However, sequence analysis of the 18S rRNA gene showed a 0.8% difference when compared to the reference E. histolytica HM-1:IMSS human strain. Differences were also observed in the 5.8S rRNA gene and the internal transcribed spacer (ITS) regions 1 and 2, and analysis of the serine-rich protein gene from the monkey strain showed unique codon usages compared to E. histolytica isolated from humans. The amino acid sequences of two hexokinases and two glucose phosphate isomerases also differed from those of E. histolytica. Isoenzyme analyses of these enzymes in the monkey strain showed different electrophoretic mobility patterns compared with E. histolytica isolates. Analysis of peroxiredoxin genes indicated the presence of at least seven different types of protein, none of which were identical to proteins in E. histolytica. When the trophozoites from the monkey strain were inoculated into the livers of hamsters, formation of amebic abscesses was observed 7 days after the injection. These results demonstrate that the strain is genetically different from E. histolytica and is virulent. Revival of the name Entamoeba nuttalli is proposed for the organism.
|
| 巻・号 |
153(2)
|
| ページ |
107-14
|
| 公開日 |
2007-6-1
|
| DOI |
10.1016/j.molbiopara.2007.02.006
|
| PII |
S0166-6851(07)00063-1
|
| PMID |
17403547
|
| MeSH |
Animals
Base Sequence
Cricetinae
DNA, Protozoan / analysis
DNA, Ribosomal / analysis
Entamoeba / classification*
Entamoeba / genetics
Entamoeba / isolation & purification
Entamoeba / pathogenicity*
Entamoeba histolytica / classification*
Entamoeba histolytica / genetics
Entamoebiasis / parasitology
Entamoebiasis / veterinary*
Humans
Liver / parasitology
Liver / pathology
Macaca mulatta / parasitology*
Male
Mesocricetus
Molecular Sequence Data
Monkey Diseases / parasitology*
RNA, Ribosomal, 18S
Virulence
|
| IF |
1.571
|
| 引用数 |
43
|
|
WOS 分野
|
PARASITOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 病原微生物 |
E. histolytica? |
