RRC ID 31715
Author Burger E, Vaz CC, Sano A, Calich VL, Singer-Vermes LM, Xidieh CF, Kashino SS, Nishimura K, Miyaji M.
Title Paracoccidioides brasiliensis infection in nude mice: studies with isolates differing in virulence and definition of their T cell-dependent and T cell-independent components.
Journal Am J Trop Med Hyg
Abstract Athymic and euthymic BALB/c mice infected with highly (Pb18) or slightly (Pb265) virulent Paracoccidioides brasiliensis isolates were compared regarding mortality, presence of viable yeasts, specific immunoglobulin M (IgM) and IgG titers, and the antigen recognition patterns of these antibodies. Isolate Pb18 caused a more severe disease in athymic mice, as supported by higher number of infected organs and shorter survival times. These animals, however, were resistant to Pb265 infection. High titers of antibodies were found only in euthymic mice, seven weeks after Pb18 infection. At this time, euthymic animals presented IgG antibodies to numerous protein bands that were not detected at four weeks postinfection or after Pb265 inoculation. In contrast, antibodies from athymic mice always reacted with few antigen bands. Although the majority of P. brasiliensis antigens are T cell-dependent, the immunodominant gp43 and also the 41.5- and 27.5-kD antigens are here, for the first time, characterized as T cell-independent antigens of P. brasiliensis.
Volume 55(4)
Pages 391-8
Published 1996-10-1
DOI 10.4269/ajtmh.1996.55.391
PMID 8916794
MeSH Animals Antibodies, Fungal / blood Antibodies, Fungal / immunology Antibody Specificity Antigens, Fungal / immunology Immunity, Cellular Immunoblotting Immunoglobulin G / blood Immunoglobulin G / immunology Immunoglobulin M / blood Immunoglobulin M / immunology Male Mice Mice, Inbred BALB C Mice, Nude Paracoccidioides / immunology Paracoccidioides / pathogenicity* Paracoccidioidomycosis / immunology* Paracoccidioidomycosis / microbiology* Paracoccidioidomycosis / mortality Specific Pathogen-Free Organisms T-Lymphocytes / physiology* Virulence Viscera / microbiology
IF 2.126
Times Cited 20
Pathogenic microorganisms IFM 41621 IFM 41629 IFM 46466