Reference - Detail
| RRC ID | 31746 |
|---|---|
| Author | Takeo K, Tanaka R, Taguchi H, Nishimura K. |
| Title | Analysis of ploidy and sexual characteristics of natural isolates of Cryptococcus neoformans. |
| Journal | Can J Microbiol |
| Abstract |
We report here an extensive examination of the ploidy of a basidiomycetous yeast. The ploidy of 34 strains of Cryptococcus neoformans var. neoformans, isolated mostly from Japanese patients, was determined by means of quantitative fluorescence microscopy after DNA staining with propidium iodide. The sexual characteristics of the strains including the two tester strains were as follows. Twenty-four strains were of the heterothallic alpha type, 1 was of the heterothallic a type, and 2 were self fertile; the remaining 7 showed no sexual response. The following strains were found to be haploid: the heterothallic a strain, 20 of the 24 heterothallic alpha strains, and 3 of the 7 asexual strains. Thus, the C. neoformans strains were mostly haploid, as the sexual response of this organism suggests. Both of the self-fertile strains and four of the seven asexual strains were diploid. Unexpectedly, 4 of the 24 heterothallic alpha strains were diploid. |
| Volume | 39(10) |
| Pages | 958-63 |
| Published | 1993-10-1 |
| DOI | 10.1139/m93-144 |
| PMID | 8261332 |
| MeSH | Cryptococcus neoformans / genetics* Cryptococcus neoformans / isolation & purification DNA, Fungal / analysis* Humans Microscopy, Fluorescence Ploidies* |
| IF | 1.793 |
| Times Cited | 19 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY IMMUNOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Pathogenic microorganisms | IFM 5807 IFM 5808 IFM 5809 IFM 5810 IFM 5811 IFM 5812 IFM 5814 IFM 5817 IFM 5818 IFM 5819 IFM 5821 IFM 5822 IFM 5824 IFM 5825 IFM 5826 IFM 5827 IFM 5829 IFM 5830 IFM 5832 IFM 5833 IFM 5836 IFM 5837 IFM 5844 IFM 5845 IFM 5854 IFM 5857 IFM 5860 IFM 40042 IFM 41465 IFM 41466 IFM 41467 IFM 41468 IFM 41469 IFM 41470 |