RRC ID 31772
著者 Imai T, Watanabe K, Tamura M, Mikami Y, Tanaka R, Nishimura K, Miyaji M, Poonwan N, Branchini ML.
タイトル Geographic grouping of Cryptococcus neoformans var. gattii by random amplified polymorphic DNA fingerprint patterns and ITS sequence divergence.
ジャーナル Clin Lab
Abstract Eleven reference strains of Cryptococcus neoformans var. gattii were analyzed by random amplified polymorphic DNA (RAPD) patterns using three oligo primers. Three major RAPD pattern profiles (profiles I, II and III) were identified with A-1 oligo primer. Each profile was found to relate to a geographic region. Since the strains which belong to profiles I and II were mainly the isolates from America, and profile III from Asia with A-1 primer, these three profiles were assigned to the geographic grouping of America-1, America-2 and Asia, respectively. Analysis of rDNA sequences coding an internal transcribed spacer (ITS) region of C. neformans var. gattii revealed that the fungus with each RAPD profile has characteristic base sequences at the four positions (10 and 15 positions in ITS1 and 8 and 56 positions at ITS2) of the ITS regions. On the basis of the combinations of the four bases specific for the ITS regions, four ITS types, AAGG (America-1), AAAC (America-2), GGGC (Asia-1) and AGGC (Asia-2) were identified: the geographic group of Asia was further classified into two subgroups of Asia-1 and Asia-2 based on the ITS typing. Clinical isolates from Thailand (6 strains) and Brazil (7 strains) were found to belong to the geographic group of America-1 nd America-2, respectively. Five reference strains of C. neoformans var. gattii from the CBS culture collection were classified into two America-2, one Asia-1 and two Asia-2 groups. This ITS region analysis allowed us to distinguish all isolates of C. neoformans var. gattii into four geographic groups based on the ITS base sequence, and further molecular epidemiological and ecological research on this fungus is recommended.
巻・号 46(7-8)
ページ 345-54
公開日 2000-1-1
PMID 10934581
MeSH Base Sequence Cryptococcus neoformans / classification* Cryptococcus neoformans / genetics* Cryptococcus neoformans / isolation & purification DNA Fingerprinting / methods* DNA, Bacterial / chemistry DNA, Bacterial / genetics Genetic Variation* Geography Humans Molecular Sequence Data Phylogeny Random Amplified Polymorphic DNA Technique* Sequence Alignment Trees / microbiology
IF 0.94
リソース情報
病原微生物 IFM 5815 IFM 5838 IFM 5851 IFM 5855 IFM 5856 IFM 5878 IFM 5880 IFM 5883 IFM 5884 IFM 40216 IFM 45713 IFM 45714 IFM 45838 IFM 46566 IFM 46914 IFM 46915 IFM 46916 IFM 47258 IFM 48213 IFM 48216 IFM 48297 IFM 48818 IFM 48819 IFM 48820 IFM 48821 IFM 48822