RRC ID 3178
Author Yamada-Noda M, Ohkusu K, Hata H, Shah MM, Nhung PH, Sun XS, Hayashi M, Ezaki T.
Title Mycobacterium species identification--a new approach via dnaJ gene sequencing.
Journal Syst. Appl. Microbiol.
Abstract The availability of the dnaJ1 gene for identifying Mycobacterium species was examined by analyzing the complete dnaJ1 sequences (approximately 1200 bp) of 56 species (54 of them were type strains) and comparing sequence homologies with those of the 16S rRNA gene and other housekeeping genes (rpoB, hsp65). Among the 56 Mycobacterium species, the mean sequence similarity of the dnaJ1 gene (80.4%) was significantly less than that of the 16S rRNA, rpoB and hsp65 genes (96.6%, 91.3% and 91.1%, respectively), indicating a high discriminatory power of the dnaJ1 gene. Seventy-one clinical isolates were correctly clustered to the corresponding type strains, showing isolates belonging to the same species. In order to propose a method for strain identification, we identified an area with a high degree of polymorphism, bordered by conserved sequences, that can be used as universal primers for PCR amplification and sequencing. The sequence of this fragment (approximately 350 bp) allows accurate species identification and may be used as a new tool for the identification of Mycobacterium species.
Volume 30(6)
Pages 453-62
Published 2007-9
DOI 10.1016/j.syapm.2007.06.003
PII S0723-2020(07)00074-4
PMID 17640840
MeSH Bacterial Typing Techniques DNA, Bacterial / analysis DNA, Bacterial / chemistry HSP40 Heat-Shock Proteins / genetics* Mycobacterium / classification* Mycobacterium / genetics Mycobacterium / isolation & purification Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 3.899
Times Cited 14
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
Pathogenic microorganisms 56 Mycobacterium species?