RRC ID 31804
Author Poonwan N, Mikami Y, Poosuwan S, Boon-Long J, Mekha N, Kusum M, Yazawa K, Tanaka R, Nishimura K, Konyama K.
Title Serotyping of Cryptococcus neoformans strains isolated from clinical specimens in Thailand and their susceptibility to various antifungal agents.
Journal Eur J Epidemiol
Abstract One hundred and thirty-nine strains of Cryptococcus neoformans were isolated in Thailand from clinical specimens including 97 AIDS patients: 67 from Northern, 48 from Central, 17 from Northeastern and 7 from Southern regional hospitals. Six out of the 139 strains were serotype B and the remaining 133 were A. There was no correlation between serotypes and regional distribution. To our knowledge, this is the first report of serotyping studies on C. neoformans in Thailand. Studies on random amplified polymorphic DNA (RAPD) analysis showed that this method is useful for the differentiation of C. neoformans var. gattii (serotypes B and C) and C. neoformans var. neoformans (serotypes A and D). They also indicated that Thai isolates of C. neoformans var. gattii (serotype B) were a homogeneous group on the basis of their genotypes. Antifungal susceptibility tests using 5 antifungal agents including amphotericin B, fluconazole, flucytosine, itraconazole and micronazole against 50 selected strains of C. neoformans showed that they were sensitive to all of the antifungal agents tested except for one strain that was resistant to flucytosine.
Volume 13(3)
Pages 335-40
Published 1997-4-1
DOI 10.1023/a:1007376917836
PMID 9258534
MeSH AIDS-Related Opportunistic Infections / microbiology Antifungal Agents / pharmacology* Cerebrospinal Fluid / microbiology Cryptococcosis / microbiology* Cryptococcus neoformans / drug effects* Cryptococcus neoformans / isolation & purification Humans Microbial Sensitivity Tests Serotyping Thailand
IF 6.529
Times Cited 35
Pathogenic microorganisms IFM 5854 IFM 5855 IFM 5856 IFM 5857