| RRC ID |
3183
|
| 著者 |
Iihara H, Niwa T, Shah MM, Nhung PH, Song SX, Hayashi M, Ohkusa K, Itoh Y, Makino S, Ezaki T.
|
| タイトル |
Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters.
|
| ジャーナル |
Jpn J Infect Dis
|
| Abstract |
An indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (LPS) of Burkholderia pseudomallei and taxonomically closely related species was developed with the Luminex system. LPSs of Pseudomonas aeruginosa, Burkholderia cepacia, Burkholderia thailandensis, Burkholderia vietnamiensis, B. pseudomallei, and Burkholderia mallei were successfully conjugated to Luminex microspheres. Antibodies measured against the LPS of B. pseudomallei-conjugated Luminex beads only cross-reacted with those of two genetically closely related species, B. mallei and B. thailandensis (previously classified as non-pathogenic arabinose-negative B. pseudomallei). However, this system could distinguish other closely related species from B. pseudomallei. This assay is able to detect significantly high levels of anti-LPS antibodies of B. pseudomallei in serum from patients with culture-proven melioidosis.
|
| 巻・号 |
60(4)
|
| ページ |
230-4
|
| 公開日 |
2007-7-1
|
| PMID |
17642542
|
| MeSH |
Antibodies, Bacterial / analysis*
Antibodies, Bacterial / immunology
Burkholderia pseudomallei / classification
Burkholderia pseudomallei / immunology*
Enzyme-Linked Immunosorbent Assay / methods
Fluorescent Antibody Technique, Indirect / methods*
Lipopolysaccharides / immunology
Melioidosis / diagnosis
Melioidosis / microbiology
Microspheres
O Antigens / immunology
|
| IF |
1.24
|
| 引用数 |
10
|
|
WOS 分野
|
INFECTIOUS DISEASES
|
| リソース情報 |
| 病原微生物 |
P. aeruginosa(GTC 00002)
B. cepacia(GTC 00013)
B. thailandensis(GTC 3P0407)
B. vietnamiensis( GTC 3P0436 )
B. pseudomallei(GTC 3P0056)
B. mallei (GTC 3P0003)?
L. pneumophila( GTC 00754 )
E. coli (GTC 00503T) |
