論文 - 詳細
| RRC ID | 31841 |
|---|---|
| 著者 | Wang L, Yokoyama K, Takahasi H, Kase N, Hanya Y, Yashiro K, Miyaji M, Nishimura K. |
| タイトル | Identification of species in Aspergillus section Flavi based on sequencing of the mitochondrial cytochrome b gene. |
| ジャーナル | Int J Food Microbiol |
| Abstract |
The partial sequences of the mitochondrial (mt) cytochrome b gene (402 bp) were determined for species of Aspergillus section Flavi. On the basis of identities of DNA sequences, 77 strains were divided into seven DNA types, from D-1 to D-7. The type strains of A. sojae, A. parasiticus, A. flavus and A. oryzae together, A. tamarii, and A. nomius were placed in DNA types D-1. D-2, D-4, D-5 and D-7, respectively. These species could be differentiated from each other. Furthermore, two other DNA types, D-3 and D-6 were found. DNA type D-3 was closely related to A. parasiticus (D-2) and included one strain that deposited as A. flatus var. flavus and produced aflatoxins B and G. DNA types D-6 included one strain named A. flavus and closely related to A. tamarii. The observations of conidial wall texture by SEM (Scanning Electron Microscopy) supported the relationships derived from the cytochrome b gene. The production of aflatoxins was also examined. Using the DNA sequence of cytochrome b gene, several strains were reidentified. The derived amino acids sequences were all the same in the studied strains. The mt cytochrome b gene is useful and reliable in distinguishing and identifying the species in Aspergillus section Flavi. |
| 巻・号 | 71(1) |
| ページ | 75-86 |
| 公開日 | 2001-12-4 |
| DOI | 10.1016/s0168-1605(01)00597-9 |
| PII | S0168-1605(01)00597-9 |
| PMID | 11764895 |
| MeSH | Amino Acid Sequence Aspergillus / classification* Aspergillus / genetics* Aspergillus / ultrastructure Base Sequence Cytochrome b Group / genetics* DNA, Fungal / genetics Microscopy, Electron, Scanning Mitochondria Molecular Sequence Data Phylogeny Polymerase Chain Reaction / methods |
| IF | 4.187 |
| 引用数 | 40 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | IFM 5366 IFM 5370 IFM 40603 IFM 40800 IFM 40801 IFM 41087 IFM 41933 IFM 42199 IFM 45909 IFM 45910 IFM 45911 IFM 46870 IFM 47030 IFM 47031 IFM 47032 IFM 47634 IFM 47636 IFM 47637 IFM 47646 IFM 47647 IFM 47648 IFM 47666 IFM 47667 IFM 47668 IFM 47669 IFM 47798 IFM 48053 IFM 48072 IFM 48073 IFM 48164 IFM 48165 IFM 48166 IFM 48479 IFM 49388 IFM 49389 IFM 49390 IFM 49393 IFM 49394 IFM 49395 IFM 49396 IFM 49398 IFM 49399 IFM 49400 IFM 49405 IFM 49406 IFM 49410 IFM 49413 IFM 49414 IFM 49415 IFM 49416 IFM 49417 IFM 49418 IFM 49419 IFM 49420 IFM 49421 IFM 49426 IFM 49427 IFM 49428 IFM 49429 IFM 49430 IFM 49431 IFM 49432 IFM 49834 IFM 49835 IFM 49836 IFM 49837 IFM 49838 IFM 49839 IFM 49840 IFM 49841 |