RRC ID 31873
Author Hussain Z, Katas H, Mohd Amin MC, Kumolosasi E.
Title Efficient immuno-modulation of TH1/TH2 biomarkers in 2,4-dinitrofluorobenzene-induced atopic dermatitis: nanocarrier-mediated transcutaneous co-delivery of anti-inflammatory and antioxidant drugs.
Journal PLoS One
Abstract The present study was conducted with the aim to investigate the immuno-modulatory and histological stabilization effects of nanocarrier-based transcutaneous co-delivery of hydrocortisone (HC) and hydroxytyrosol (HT). In this investigation, the clinical and pharmacological efficacies of nanoparticle (NP)-based formulation to alleviate 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD) was explored by using an NC/Nga mouse model. Ex vivo visual examination of AD induction in experimental mice indicated remarkable control of NP-based formulations in reducing pathological severity of AD-like skin lesions. Therapeutic effectiveness of NP-based formulations was also evaluated by comparing skin thickness of AD-induced NP-treated mice (456±27 µm) with that of atopic mice (916±37 µm). Analysis of the immuno-spectrum of AD also revealed the dominance of NP-based formulations in restraining immunoglobulin-E (IgE), histamine, prostaglandin-E2 (PGE2), vascular endothelial growth factor-α (VEGF-α), and T-helper cells (TH1/TH2) producing cytokines in serum and skin biopsies of tested mice. These anti-AD data were further supported by histological findings that revealed alleviated pathological features, including collagen fiber deposition, fibroblasts infiltration, and fragmentation of elastic fibers in experimental mice. Thus, NP-mediated transcutaneous co-delivery of HC and HT can be considered as a promising therapy for managing immunological and histological spectra associated with AD.
Volume 9(11)
Pages e113143
Published 2014-1-1
DOI 10.1371/journal.pone.0113143
PII PONE-D-14-22182
PMID 25396426
PMC PMC4232601
MeSH Administration, Cutaneous Animals Anti-Inflammatory Agents / administration & dosage* Antioxidants / administration & dosage* Biomarkers / metabolism Cytokines / blood Dermatitis, Atopic / chemically induced Dermatitis, Atopic / drug therapy* Dermatitis, Atopic / pathology Dinitrofluorobenzene / toxicity Dinoprostone / analysis Dinoprostone / blood Disease Models, Animal Drug Carriers / chemistry Histamine / analysis Histamine / blood Hydrocortisone / administration & dosage Immunoglobulin E / analysis Immunoglobulin E / blood Mice Nanoparticles / chemistry* Phenylethyl Alcohol / administration & dosage Phenylethyl Alcohol / analogs & derivatives Skin / pathology Th1 Cells / immunology Th1 Cells / metabolism* Th2 Cells / immunology Th2 Cells / metabolism* Vascular Endothelial Growth Factor A / analysis Vascular Endothelial Growth Factor A / blood
IF 2.74
Times Cited 14
Mice RBRC01059