RRC ID 3192
Author Masuda Y, Kamiya K.
Title Biochemical properties of the human REV1 protein.
Journal FEBS Lett
Abstract It has been proposed that the REV1 protein plays an important role in the induced-mutagenesis pathway. We show that purified REV1 protein inserts dCMP opposite template G, A, T and C, and dGMP and dTMP opposite template G in the presence of magnesium, while in the presence of manganese the specificity for dCMP was found to be relaxed and the REV1 protein acquired the ability to insert dCMP, dGMP, dAMP and dTMP opposite templates G, A, T, and C. Kinetic analysis provided evidence for high affinity for dCTP with template G, suggesting that the REV1 protein is specialized for dCTP and template G.
Volume 520(1-3)
Pages 88-92
Published 2002-6-5
DOI 10.1016/s0014-5793(02)02773-4
PII S0014579302027734
PMID 12044876
MeSH Base Sequence Cations, Divalent / pharmacology Deoxycytidine Monophosphate / metabolism Dose-Response Relationship, Drug Escherichia coli / genetics Gene Expression Humans Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Nuclear Proteins Nucleotidyltransferases / drug effects Nucleotidyltransferases / genetics Nucleotidyltransferases / metabolism* Plasmids / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / isolation & purification Recombinant Fusion Proteins / metabolism
IF 3.057
Times Cited 30
Prokaryotes E. coli ME9042(BL21(DE3))