論文 - 詳細
| RRC ID | 3192 |
|---|---|
| 著者 | Masuda Y, Kamiya K. |
| タイトル | Biochemical properties of the human REV1 protein. |
| ジャーナル | FEBS Lett |
| Abstract |
It has been proposed that the REV1 protein plays an important role in the induced-mutagenesis pathway. We show that purified REV1 protein inserts dCMP opposite template G, A, T and C, and dGMP and dTMP opposite template G in the presence of magnesium, while in the presence of manganese the specificity for dCMP was found to be relaxed and the REV1 protein acquired the ability to insert dCMP, dGMP, dAMP and dTMP opposite templates G, A, T, and C. Kinetic analysis provided evidence for high affinity for dCTP with template G, suggesting that the REV1 protein is specialized for dCTP and template G. |
| 巻・号 | 520(1-3) |
| ページ | 88-92 |
| 公開日 | 2002-6-5 |
| DOI | 10.1016/s0014-5793(02)02773-4 |
| PII | S0014579302027734 |
| PMID | 12044876 |
| MeSH | Base Sequence Cations, Divalent / pharmacology Deoxycytidine Monophosphate / metabolism Dose-Response Relationship, Drug Escherichia coli / genetics Gene Expression Humans Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Nuclear Proteins Nucleotidyltransferases / drug effects Nucleotidyltransferases / genetics Nucleotidyltransferases / metabolism* Plasmids / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / isolation & purification Recombinant Fusion Proteins / metabolism |
| IF | 3.057 |
| 引用数 | 30 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9042(BL21(DE3)) |