| RRC ID |
31920
|
| 著者 |
Kurita N, Sano A, Coelho KI, Takeo K, Nishimura K, Miyaji M.
|
| タイトル |
An improved culture medium for detecting live yeast phase cells of Paracoccidioides brasiliensis.
|
| ジャーナル |
J Med Vet Mycol
|
| Abstract |
The plating efficiency of standard mycological media such as brain heart infusion (BHI) agar is poor for Paracoccidioides brasiliensis. We prepared a water-extract of yeast phase cells of P. brasiliensis and examined it for growth-enhancing activity for the fungus. The water-extract, when added to BHI agar to a concentration of 5%, improved the plating efficiency of the medium for the fungus to some extent, but the degree of improvement was considerably varied among P. brasiliensis isolates. By contrast, when the water-extract was added in combination with horse serum (4%), the plating efficiency was highly improved (to 94-99%) for all the P. brasiliensis isolates employed. The growth-enhancing factor(s) in the water-extract was heat-stable and heating at 120 degrees C for 15 min had little, if any, effect on growth-enhancing activity.
|
| 巻・号 |
31(3)
|
| ページ |
201-5
|
| 公開日 |
1993-1-1
|
| DOI |
10.1080/02681219380000251
|
| PMID |
8360811
|
| MeSH |
Culture Media*
Paracoccidioides / growth & development*
Paracoccidioides / isolation & purification
|
| 引用数 |
30
|
|
WOS 分野
|
MYCOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 41620
IFM 41621
IFM 41624
IFM 41626
IFM 41628
IFM 41633 |
