論文 - 詳細
| RRC ID | 31922 |
|---|---|
| 著者 | Tanaka R, Taguchi H, Takeo K, Miyaji M, Nishimura K. |
| タイトル | Determination of ploidy in Cryptococcus neoformans by flow cytometry. |
| ジャーナル | J Med Vet Mycol |
| Abstract |
We determined the ploidy of Cryptococcus neoformans (28 strains) isolated from patients and nature. The cellular DNA content of these strains, which stained with propidium iodide in comparison to that of two authentic haploid strains, was determined by flow cytometry. All the strains exhibited diphasic histograms. In case of the authentic haploid strains, the first peak was centred around channel 9, and the second peak around channel 18. Most strains exhibited this type of histogram. Some strains exhibited another type of histogram: the first peak was centred around channel 18, and the second one around channel 35. In flow cytometry, the channel number is correlated with the intensity of fluorescence, namely, in proportion to the channel number the DNA content in the cells increases. The cellular DNA content of the second type of histograms showed twice that of the authentic haploid strains, and thus, five of 28 isolates were concluded to be diploid, and the others haploid. |
| 巻・号 | 34(5) |
| ページ | 299-301 |
| 公開日 | 1996-1-1 |
| PMID | 8912162 |
| MeSH | Cryptococcosis / microbiology Cryptococcus neoformans / cytology Cryptococcus neoformans / genetics* Cryptococcus neoformans / isolation & purification DNA, Fungal / analysis Flow Cytometry Humans Ploidies* |
| 引用数 | 41 |
| WOS 分野 | MYCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | IFM 5807 IFM 5808 IFM 5809 IFM 5810 IFM 5811 IFM 5812 IFM 5813 IFM 5814 IFM 5817 IFM 5818 IFM 5821 IFM 5822 IFM 5824 IFM 5826 IFM 5827 IFM 5829 IFM 5831 IFM 5833 IFM 5837 IFM 5844 IFM 5845 IFM 40042 IFM 41463 IFM 41464 IFM 41466 IFM 41468 IFM 41469 IFM 41470 |