Reference - Detail
| RRC ID | 31924 |
|---|---|
| Author | Kucsera J, Yarita K, Takeo K. |
| Title | Simple detection method for distinguishing dead and living yeast colonies. |
| Journal | J Microbiol Methods |
| Abstract |
A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. |
| Volume | 41(1) |
| Pages | 19-21 |
| Published | 2000-6-1 |
| DOI | 10.1016/s0167-7012(00)00136-6 |
| PII | S0167-7012(00)00136-6 |
| PMID | 10856773 |
| MeSH | Basidiomycota / cytology* Basidiomycota / drug effects Candida albicans / cytology* Candida albicans / drug effects Coloring Agents / toxicity Gentian Violet / toxicity Methylene Blue / toxicity Rose Bengal / toxicity Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / drug effects Schizosaccharomyces / cytology* Schizosaccharomyces / drug effects Trypan Blue / toxicity |
| IF | 1.707 |
| Times Cited | 52 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS MICROBIOLOGY |
| Resource | |
| Pathogenic microorganisms | IFM 40009 IFM 40022 IFM 40078 IFM 40190 |