論文 - 詳細
| RRC ID | 31940 |
|---|---|
| 著者 | Higa M, Kita A, Hagihara K, Kitai Y, Doi A, Nagasoko R, Satoh R, Sugiura R. |
| タイトル | Spatial control of calcineurin in response to heat shock in fission yeast. |
| ジャーナル | Genes Cells |
| Abstract |
In fission yeast, Ppb1, the Ca2+/calmodulin-dependent protein phosphatase calcineurin regulates multiple biological processes, such as cytokinesis, Ca2+-homeostasis, membrane trafficking and cell wall integrity. Calcineurin dephosphorylates the Prz1 transcription factor, leading to its nuclear translocation and gene expression under the control of CDRE (calcineurin-dependent response element). Although the calcineurin-mediated spatial control of downstream transcription factors has been intensively studied in many organisms, less is known about the spatial regulation of calcineurin on stresses. Here, we show that heat shock stimulates calcineurin-dependent nuclear translocation of Prz1 and CDRE-dependent gene expression. Notably, calcineurin exhibited a dramatic change in subcellular localization, translocating from diffuse cytoplasmic to dot-like structures on heat shock. The calcineurin dots colocalized with Dcp2 or Pabp, the constituent of P-bodies or stress granules, respectively, thus suggesting that calcineurin is a component of RNA granules under heat shock. Importantly, the calcineurin inhibitor FK506 markedly inhibited the accumulation of calcineurin granules, whereas the constitutively active calcineurin strongly accumulated in the granules on heat shock, suggesting that phosphatase activity is important for calcineurin localization. Notably, the depletion of calcineurin induced a rapid appearance of Nrd1- and Pabp-positive RNA granules. The possible roles of calcineurin in response to heat shock will be discussed. |
| 巻・号 | 20(2) |
| ページ | 95-107 |
| 公開日 | 2015-2-1 |
| DOI | 10.1111/gtc.12203 |
| PMID | 25529221 |
| MeSH | Calcineurin / chemistry Calcineurin / metabolism* Calcineurin Inhibitors / pharmacology Cycloheximide / pharmacology Gene Expression Heat-Shock Response* Protein Synthesis Inhibitors / pharmacology Protein Transport / drug effects Ribonucleoproteins / metabolism* Ribonucleoproteins / ultrastructure Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / metabolism* Tacrolimus / pharmacology Transcription Factors / metabolism |
| IF | 1.655 |
| 引用数 | 2 |
| WOS 分野 | CELL BIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY9899? |