| RRC ID |
31943
|
| 著者 |
Hamano K, Ueno-Tsuji S, Tanaka R, Suzuki M, Nishimura K, Nishigaki K.
|
| タイトル |
Genome profiling (GP) as an effective tool for monitoring culture collections: a case study with Trichosporon.
|
| ジャーナル |
J Microbiol Methods
|
| Abstract |
Species identification and classification of a large number of microbes are essential and heavy workloads in culture collections and relevant laboratories. The identification of species usually requires different methods depending on species. Therefore, the development of a method which is simple and applicable to any organisms will lessen the burdens, increase the reliability of databases and thus enhance the science on microbes. The genome profiling (GP) method, developed previously, was found effective in monitoring authenticities of all strains/species tested in culture collections and expectedly various species, which was shown by applying the GP and the conventional sequencing methods to identifying and classifying species/strains belonging to the genus Trichosporon (38 strains; 16 species). Small differences between strains (11 strains of Trichosporon asahii and 4 strains of Trichosporon coremiiforme) can be reliably discriminated by GP, which was unsuccessful in the conventional sequencing approach. Importantly, seven possible false-assignments contained in the database were all pointed out by the GP method with near-perfect correctness, showing the power of the GP method.GP was shown to be a potent tool for rapidly and correctly monitoring species and strains of fungi in culture collections owing to its simple and informative natures.
|
| 巻・号 |
89(2)
|
| ページ |
119-28
|
| 公開日 |
2012-5-1
|
| DOI |
10.1016/j.mimet.2012.02.007
|
| PII |
S0167-7012(12)00061-9
|
| PMID |
22401825
|
| MeSH |
Biological Specimen Banks*
DNA Fingerprinting / methods*
DNA, Fungal / chemistry
DNA, Fungal / genetics
Diagnostic Errors
Genome, Fungal*
Molecular Sequence Data
Sensitivity and Specificity
Sequence Analysis, DNA
Trichosporon / classification*
Trichosporon / genetics*
|
| IF |
1.707
|
| 引用数 |
6
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| 病原微生物 |
IFM 40140
IFM 41129
IFM 41606
IFM 48429
IFM 48551
IFM 48558
IFM 48559
IFM 48560
IFM 48563
IFM 48564
IFM 48565
IFM 48575
IFM 48577
IFM 48578
IFM 48579
IFM 48581
IFM 48582
IFM 48608
IFM 48609
IFM 48611
IFM 48620
IFM 48960
IFM 48961
IFM 48963
IFM 49226
IFM 50223
IFM 50224
IFM 51250
IFM 51597
IFM 51598
IFM 52660
IFM 52822
IFM 53544
IFM 53746
IFM 53856
IFM 53858
IFM 53879 |
| 一般微生物 |
JCM 1541
JCM 1542 |
