Reference - Detail
| RRC ID | 31979 |
|---|---|
| Author | Harwood BN, Draper I, Kopin AS. |
| Title | Targeted inactivation of the rickets receptor in muscle compromises Drosophila viability. |
| Journal | J Exp Biol |
| Abstract |
Bursicon is a hormone that modulates wing expansion, cuticle hardening and melanization in Drosophila melanogaster. Bursicon activity is mediated through its cognate G protein-coupled receptor (GPCR), rickets. We have developed a membrane-tethered bursicon construct that enables spatial modulation of rickets-mediated physiology in transgenic flies. Ubiquitous expression of tethered bursicon throughout development results in arrest at the pupal stage. The few organisms that eclose fail to undergo wing expansion. These phenotypes suggest that expression of tethered bursicon inhibits rickets-mediated function. Consistent with this hypothesis, we show in vitro that sustained stimulation of rickets by tethered bursicon leads to receptor desensitization. Furthermore, tissue-specific expression of the tethered bursicon inhibitor unraveled a critical role for rickets in a subset of adult muscles. Taken together, our findings highlight the utility of membrane-tethered inhibitors as important genetic/pharmacological tools to dissect the tissue-specific roles of GPCRs in vivo. |
| Volume | 217(Pt 22) |
| Pages | 4091-8 |
| Published | 2014-11-15 |
| DOI | 10.1242/jeb.110098 |
| PII | jeb.110098 |
| PMID | 25278473 |
| PMC | PMC4495466 |
| MeSH | Animals Animals, Genetically Modified Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Insect Hormones / metabolism Invertebrate Hormones / metabolism* Invertebrate Hormones / physiology* Metamorphosis, Biological / physiology* Muscles / physiology* Receptors, G-Protein-Coupled / metabolism* |
| IF | 3.014 |
| Times Cited | 4 |
| WOS Category | BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 8930R-1 |