| RRC ID |
32175
|
| 著者 |
Hata K, Ohkusu M, Aoki S, Ito-Kuwa S, Pienthaweechai K, Takeo K.
|
| タイトル |
Cells of different ploidy are often present together in Cryptococcus neoformans strains.
|
| ジャーナル |
Nihon Ishinkin Gakkai Zasshi
|
| Abstract |
We examined the ploidy of C. neoformans strains using both laser scanning cytometry and a fluorescence microscope equipped with a photomultiplier. Haploid strains consisted of normal-sized cells with a haploid DNA amount. In the cell population there were also large-sized cells with a diploid DNA amount. These large cells in haploid strains were isolated using a Skerman micromanipulator, cultivated, and were able to generate diploid clones. Even after only 3-5 transfers on slants, haploid cells were present in all the diploid clones examined. Conversely, haploid clones obtained by single-cell-isolation of normal-sized cells from haploid strains were also shown to contain diploid cells after 3-5 transfers. Fresh haploid isolates from the environment similarly contained diploid cells after 3-5 transfers. Thus, a cellular ploidy shift was shown to occur widely in C. neoformans strains.
|
| 巻・号 |
41(3)
|
| ページ |
161-7
|
| 公開日 |
2000-1-1
|
| DOI |
10.3314/jjmm.41.161
|
| PMID |
10938517
|
| MeSH |
Cryptococcus neoformans / cytology*
DNA, Fungal / analysis
Microscopy, Fluorescence
Ploidies*
|
| リソース情報 |
| 病原微生物 |
IFM 5807
IFM 5823
IFM 5824
IFM 5835
IFM 40038
IFM 45941
IFM 47847
IFM 47862
IFM 47877
IFM 48184
IFM 48313
IFM 48314
IFM 48484
IFM 48711
IFM 48764
IFM 48908
IFM 48909
IFM 48941
IFM 48943
IFM 49026
IFM 49085
IFM 49087
IFM 49088
IFM 49120
IFM 49121
IFM 49137
IFM 49160
IFM 49177 |
