論文 - 詳細
| RRC ID | 32177 |
|---|---|
| 著者 | Biswas SK, Yokoyama K, Wang L, Nishimura K, Miyaji M. |
| タイトル | Identification of Candida dubliniensis based on the specific amplification of mitochondrial cytochrome b gene. |
| ジャーナル | Nihon Ishinkin Gakkai Zasshi |
| Abstract |
Candida dubliniensis, a recently described Candida species, is frequently isolated from oral candidiasis in human immunodeficiency virus infected individuals. To detect the organism rapidly, we have developed specific oligonucleotide primers based on the sequences of mitochondrial cytochrome b gene. These primers selectively amplified DNA only from C. dubliniensis; the DNAs of all pathogenic Candida species tested, as well as those of medically relevant yeasts such as Cryptococcus neoformans, and Trichosporon cutaneum, were not amplified. This is the first report describing the effectiveness of cytochrome b gene in PCR based detection of an organism, and we hope the system will be useful as a microbiological tool for rapid detection of C. dubliniensis. |
| 巻・号 | 42(2) |
| ページ | 95-8 |
| 公開日 | 2001-1-1 |
| DOI | 10.3314/jjmm.42.95 |
| PMID | 11331471 |
| MeSH | Base Sequence Candida / isolation & purification* Candidiasis, Oral / microbiology Cytochrome b Group / genetics* DNA Primers Humans Mitochondria / enzymology Molecular Sequence Data Polymerase Chain Reaction |
| リソース情報 | |
| 病原微生物 | IFM 5464 IFM 5489 IFM 5728 IFM 5772 IFM 40019 IFM 40021 IFM 40090 IFM 40117 IFM 40140 IFM 46462 IFM 46816 IFM 46823 IFM 46829 IFM 46834 IFM 46841 IFM 46843 IFM 48184 IFM 48311 IFM 48391 IFM 48637 IFM 48776 IFM 48922 IFM 49108 IFM 49206 IFM 49207 IFM 49209 IFM 49689 IFM 49723 IFM 48313 IFM 48314 |