RRC ID 32182
Author Tanabe H, Kawasaki M, Mochizuki T, Ishizaki H.
Title Species identification and strain typing of Fonsecaea pedrosoi using ribosomal RNA gene internal transcribed spacer regions.
Journal Nihon Ishinkin Gakkai Zasshi
Abstract The restriction fragment length polymorphism (RFLP) in the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA gene (rDNA) was analyzed on Fonsecaea pedrosoi isolates kept in the Department of Dermatology, Kanazawa Medical University, Japan. On the bases of the RFLP patterns with Dde I and Msp I, 131 isolates were classified into 5 types (D1-D5) and 4 types (M1-M4), respectively. Combining the RFLP patterns with Dde I and Msp I, the isolates were further classified into 6 rDNA-types which corresponded to the 6 mtDNA-types reported by Kawasaki et al. based on the mtDNA-RFLP patterns, except for a single strain of mtDNA-type 7, which was indistinguishable from mtDNA-type 2. The strains of each rDNA-type formed a clade on the phylogenetic tree constructed from sequences of the ITS regions. ITS-RFLP analysis discriminated F. pedrosoi from 11 other species of pathogenic phaeoid fungi except F. compacta. These results strongly suggest that the typing based on ITS-RFLP is reliable and that F. pedrosoi and F. compacta are conspecific. Compared with mtDNA-RFLP analysis, ITS-RFLP analysis is less tedious, permits simultaneous analysis of many samples and gives equivalent results rapidly. This analysis is therefore useful for typing or epidemiologically investigating F. pedrosoi and for differentiating it from other dematiaceous fungi.
Volume 45(2)
Pages 105-12
Published 2004
PMID 15118668
MeSH Ascomycota / isolation & purification* Base Sequence Mycological Typing Techniques / methods* Polymorphism, Restriction Fragment Length RNA, Fungal / genetics* RNA, Ribosomal / genetics*
Pathogenic microorganisms IFM 4885 IFM 4886 IFM 4887 IFM 4889 IFM 4912 IFM 4913 IFM 4914 IFM 4915 IFM 4916 IFM 4917 IFM 4918 IFM 4924 IFM 4925 IFM 4931 IFM 5070 IFM 41517 IFM 41518 IFM 41519 IFM 41520 IFM 41521 IFM 41522 IFM 41523 IFM 41524 IFM 41525 IFM 41526 IFM 41527 IFM 41528 IFM 41705 IFM 41706 IFM 41867 IFM 41868 IFM 45991 IFM 45992 IFM 45993 IFM 45994 IFM 46418 IFM 46419 IFM 46420 IFM 46421 IFM 46422 IFM 46423 IFM 46424 IFM 46425 IFM 46426 IFM 46427 IFM 46428 IFM 46429 IFM 46430 IFM 46431 IFM 46432 IFM 46433 IFM 46434 IFM 46435 IFM 46436 IFM 46437 IFM 46439 IFM 46441