| RRC ID |
32186
|
| Author |
Kang Y, Iida S, Yamamoto S, Kogure T, Tanaka R, Mikami Y.
|
| Title |
Trf4 is a useful gene for discrimination of Candida tropicalis from other medically important Candida species.
|
| Journal |
Nihon Ishinkin Gakkai Zasshi
|
| Abstract |
Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, C. krusei, C. parapsilosis, or C. zeylanoides. Usefulness of the PCR primer in differentiating from clinical isolates of other fungal species is discussed.
|
| Volume |
49(1)
|
| Pages |
39-43
|
| Published |
2008-1-1
|
| DOI |
10.3314/jjmm.49.39
|
| PII |
JST.JSTAGE/jjmm/49.39
|
| PMID |
18277050
|
| MeSH |
Candida tropicalis / genetics*
Candida tropicalis / growth & development
Candida tropicalis / isolation & purification*
DNA Primers
DNA, Fungal / genetics
DNA-Directed DNA Polymerase
Genes, Fungal / genetics*
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
Saccharomyces cerevisiae Proteins
Sequence Analysis, DNA
|
| Resource |
| Pathogenic microorganisms |
IFM 5210
IFM 5340
IFM 5448
IFM 5450
IFM 5471
IFM 5474
IFM 40090
IFM 40123
IFM 46816
IFM 46921
IFM 51992
IFM 53846
IFM 53858 |
