Reference - Detail
| RRC ID | 3218 |
|---|---|
| Author | Jiao Z, Baba T, Mori H, Shimizu K. |
| Title | Analysis of metabolic and physiological responses to gnd knockout in Escherichia coli by using C-13 tracer experiment and enzyme activity measurement. |
| Journal | FEMS Microbiol Lett |
| Abstract |
The physiological and metabolic responses to gnd knockout in Escherichia coli K-12 was quantitatively investigated by using the (13)C tracer experiment (GC-MS/NMR) together with the enzyme activity analysis. It was shown that the general response to the gene knockout was the local flux rerouting via Entner-Doudoroff pathway and the direction reversing via non-oxidative pentose phosphate pathway (PPP). The mutant was found to direct higher flux to phosphoglucose isomerase reaction as compared to the wild-type, but the respiratory metabolism was comparable in both strains. The anaplerotic pathway catalyzed by malic enzyme was identified in the mutant, which was accompanied with an up-regulation of phosphoenolpyruvate carboxylase and down-regulation of phosphoenolpyruvate carboxykinase. The presented results provide first evidence that compensatory mechanism existed in PPP and anaplerotic pathway in response to the gnd deletion. |
| Volume | 220(2) |
| Pages | 295-301 |
| Published | 2003-3-28 |
| DOI | 10.1016/S0378-1097(03)00133-2 |
| PII | S0378109703001332 |
| PMID | 12670695 |
| MeSH | Carbon Isotopes Enzymes / metabolism Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli / physiology* Gene Deletion Gene Expression Regulation, Bacterial Genes, Bacterial Genetic Complementation Test Models, Biological Pentose Phosphate Pathway / physiology Phosphogluconate Dehydrogenase / genetics* Radioactive Tracers |
| IF | 1.987 |
| Times Cited | 23 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | JW2011 |