RRC ID 32194
Author Kurita N, Oarada M, Miyaji M, Ito E.
Title Effect of cytokines on antifungal activity of human polymorphonuclear leucocytes against yeast cells of Paracoccidioides brasiliensis.
Journal Med. Mycol.
Abstract In our previous study, it was observed that human peripheral blood polymorphonuclear leucocytes (PMNs) exhibited a fungistatic effect on yeast cells of Paracoccidioides brasiliensis, and that interferon-gamma (IFN-gamma), but not tumor necrosis factor-alpha or interleukin-8 (IL-8), enhanced the antifungal activity of PMNs. In the present study, granulocyte-macrophage colony-stimulating factor (GM-CSF) also enhanced the PMN activity. GM-CSF-activated PMNs exhibited a killing effect on P. brasiliensis isolate Bt-4 and an enhanced fungistatic effect on isolate Aoki. IL-1beta activated PMNs to kill isolate Bt-4. Granulocyte colony-stimulating factor had no effect. Combinations of IFN-gamma with GM-CSF or IL-1beta, but not a combination of GM-CSF and IL-1beta, exhibited a synergistic effect in enhancing the antifungal activity of PMNs. These results strongly suggest that PMNs activated with IFN-gamma, GM-CSF and/or IL-1beta might play an important role in host defense in early infection with P. brasiliensis due to their enhanced antifungal activity.
Volume 38(2)
Pages 177-82
Published 2000-4
PMID 10817235
MeSH Colony Count, Microbial Cytokines / pharmacology* Drug Synergism Granulocyte Colony-Stimulating Factor / pharmacology Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology Humans Interferon-gamma / pharmacology Interleukin-1 / pharmacology Neutrophil Activation / drug effects Neutrophils / drug effects Neutrophils / immunology* Paracoccidioides / immunology* Time Factors
IF 2.799
Times Cited 20
Pathogenic microorganisms IFM 41624