RRC ID 32196
Author Imai T, Sano A, Mikami Y, Watanabe K, Aoki FH, Branchini ML, Negroni R, Nishimura K, Miyaji M.
Title A new PCR primer for the identification of Paracoccidioides brasiliensis based on rRNA sequences coding the internal transcribed spacers (ITS) and 5 x 8S regions.
Journal Med. Mycol.
Abstract Internal transcribed spacer (ITS) genes including the 5.8S ribosomal (r)RNA of Paracoccidioides brasiliensis were amplified and the DNA sequences were determined. Based on a comparison of the sequence information, a new polymerase chain reaction (PCR) primer pair was designed for specific amplification of DNA for P. brasiliensis. This primer pair amplified a 418-bp DNA sequence and was 100% successful in identifying 29 strains of P. brasiliensis (including the reference strains) isolated from the regions of Brazil, Costa Rica, Japan, Argentina or from different sources. The results of specificity tests of these primers to compare the fungus with those of Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Histoplasma capsulatum and Penicillium marneffei are also reported.
Volume 38(4)
Pages 323-6
Published 2000-8
PMID 10975701
MeSH DNA Primers* DNA, Intergenic DNA, Ribosomal* Humans Molecular Sequence Data Paracoccidioides / genetics Paracoccidioides / isolation & purification* Polymerase Chain Reaction / methods* RNA, Ribosomal, 5.8S
IF 2.799
Times Cited 22
Pathogenic microorganisms IFM 41620 IFM 41621 IFM 41622 IFM 41623 IFM 41624 IFM 41625 IFM 41626 IFM 41628 IFM 41629 IFM 41630 IFM 41632 IFM 41633 IFM 46215 IFM 46463 IFM 46464 IFM 46465 IFM 46466 IFM 46467 IFM 46468 IFM 46470 IFM 46930