| RRC ID |
32196
|
| 著者 |
Imai T, Sano A, Mikami Y, Watanabe K, Aoki FH, Branchini ML, Negroni R, Nishimura K, Miyaji M.
|
| タイトル |
A new PCR primer for the identification of Paracoccidioides brasiliensis based on rRNA sequences coding the internal transcribed spacers (ITS) and 5 x 8S regions.
|
| ジャーナル |
Med Mycol
|
| Abstract |
Internal transcribed spacer (ITS) genes including the 5.8S ribosomal (r)RNA of Paracoccidioides brasiliensis were amplified and the DNA sequences were determined. Based on a comparison of the sequence information, a new polymerase chain reaction (PCR) primer pair was designed for specific amplification of DNA for P. brasiliensis. This primer pair amplified a 418-bp DNA sequence and was 100% successful in identifying 29 strains of P. brasiliensis (including the reference strains) isolated from the regions of Brazil, Costa Rica, Japan, Argentina or from different sources. The results of specificity tests of these primers to compare the fungus with those of Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Histoplasma capsulatum and Penicillium marneffei are also reported.
|
| 巻・号 |
38(4)
|
| ページ |
323-6
|
| 公開日 |
2000-8-1
|
| DOI |
10.1080/mmy.38.4.323.326
|
| PMID |
10975701
|
| MeSH |
DNA Primers*
DNA, Intergenic
DNA, Ribosomal*
Humans
Molecular Sequence Data
Paracoccidioides / genetics
Paracoccidioides / isolation & purification*
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 5.8S
|
| IF |
2.822
|
| 引用数 |
25
|
|
WOS 分野
|
VETERINARY SCIENCES
MYCOLOGY
INFECTIOUS DISEASES
|
| リソース情報 |
| 病原微生物 |
IFM 41620
IFM 41621
IFM 41622
IFM 41623
IFM 41624
IFM 41625
IFM 41626
IFM 41628
IFM 41629
IFM 41630
IFM 41632
IFM 41633
IFM 46215
IFM 46463
IFM 46464
IFM 46465
IFM 46466
IFM 46467
IFM 46468
IFM 46470
IFM 46930 |
