RRC ID 32208
Author Takayama A, Itano EN, Sano A, Ono MA, Kamei K.
Title An atypical Paracoccidioides brasiliensis clinical isolate based on multiple gene analysis.
Journal Med. Mycol.
Abstract An atypical isolate of Paracoccidioides brasiliensis (IFM54648), recovered from the sputum of a Brazilian man, was not detected in immunodiffusion tests for paracoccidioidomycosis and in species-specific PCR for the major antigen 43-kDa glycoprotein coding gene (gp43). The mycological characteristics of the isolate were similar to those of a typical P. brasiliensis. A total of 8 genes were sequenced from IFM54648, and the sequences were compared between the new isolate and other reference isolates and database sequences. We analyzed fragments of the gene sequences that code for gp43, the internal transcribed spacer regions of ribosomal RNA, the D1/D2 domains of the large subunit ribosomal RNA, glucan synthase, chitin synthase, glyoxalase I mRNA, 70-kDa heat-shock protein mRNA and urease. The gene sequences were 98.9-100% identical between IFM54648 and Pb01 (another atypical isolate). When compared to the other typical isolates, the identities were generally lower than 98%. A phylogenetic tree constructed using gp43 sequences showed that IFM54648 clustered with Pb01 at a considerable distance from other isolates. Therefore, this isolate is likely related to Pb01, which has recently been shown to be genetically distinct from other isolates of this species.
Volume 48(1)
Pages 64-72
Published 2010-2
DOI 10.3109/13693780902718065
PII 908828556
PMID 19225977
MeSH Brazil Cluster Analysis DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics Fungal Proteins / genetics Genes, rRNA Humans Immunodiffusion / methods Male Middle Aged Molecular Sequence Data Paracoccidioides / classification* Paracoccidioides / genetics* Paracoccidioides / isolation & purification Paracoccidioidomycosis / microbiology* Phylogeny Polymerase Chain Reaction / methods RNA, Fungal / genetics RNA, Ribosomal / genetics Sequence Analysis, DNA
IF 2.799
Times Cited 8
Pathogenic microorganisms IFM 41620 IFM 41621 IFM 41622 IFM 41623 IFM 41624 IFM 41625 IFM 41626 IFM 41627 IFM 41629 IFM 41630 IFM 41631 IFM 41632 IFM 41633 IFM 46215 IFM 46240 IFM 46463 IFM 46464 IFM 46465 IFM 46466 IFM 46467 IFM 46468 IFM 46469 IFM 46470 IFM 46930 IFM 47183 IFM 47633 IFM 50887 IFM 52933 IFM 54647 IFM 54648 IFM 54649 IFM 54650 IFM 54651 IFM 54652 IFM 54653