RRC ID 32209
Author Iwasawa MT, Togawa Y, Akita F, Kambe N, Matsue H, Yaguchi T, Nishimura K.
Title Kerion celsi due to Arthroderma incurvatum infection in a Sri Lankan child: species identification and analysis of area-dependent genetic polymorphism.
Journal Med. Mycol.
Abstract A three-year-old Sri Lankan boy residing in Japan developed a nodule on his scalp after visiting Sri Lanka. Two months later, the lesion increased in size to 22 × 19 mm(2), and was identified as an erythematous nodule with alopecia. Direct examination of the infected hair shafts indicated fungal hyphae outside the shafts. The fungus was identified as Microsporum gypseum following mycological examination. The sequence of the internal transcribed spacer 1 region of ribosomal RNA gene (ITS1 rDNA) exhibited 95.7-100.0% homology with that of Arthroderma incurvatum. The patient was successfully treated with a 6-week itraconazole course. We also examined DNA samples from eight clinical isolates of A. incurvatum. Alignments of ITS1 sequences of these strains and our isolate, showed gaps in the 64-bp positions 140-142 and 141-143 of the 205-207-bp ITS1 alignment. We performed phylogenetic analysis using the neighbor-jointing (NJ) method based on the ITS1 sequences of the present isolate and twenty related strains. Fifteen A. incurvatum strains were divided into East Asia and non-East Asia clusters. The present isolate belonged to the non-East Asia cluster, suggesting that the patient was infected outside Japan. Moreover, the trees suggested area-dependent genetic polymorphism of A. incurvatum.
Volume 50(7)
Pages 690-8
Published 2012-10
DOI 10.3109/13693786.2012.671968
PMID 22443310
MeSH Antifungal Agents / administration & dosage Arthrodermataceae / isolation & purification* Base Sequence Child, Preschool Cluster Analysis DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics Humans Itraconazole / administration & dosage Japan Male Microscopy Molecular Sequence Data Phylogeography* Polymorphism, Genetic* Scalp / pathology Sequence Alignment Sequence Analysis, DNA Sri Lanka Tinea Capitis / diagnosis* Tinea Capitis / microbiology* Treatment Outcome
IF 2.799
Times Cited 4
Pathogenic microorganisms IFM 5305 IFM 5306 IFM 5308 IFM 5309 IFM 5310 IFM 46456 IFM 46457 IFM 46928 IFM 47488 IFM 47490 IFM 47491 IFM 47492 IFM 48151 IFM 48152 IFM 49903 IFM 49904 IFM 53877 IFM 53930 IFM 59508
General Microbes JCM 1905 JCM 1906