論文 - 詳細
| RRC ID | 32214 |
|---|---|
| 著者 | Ito Y, Hirasawa T, Shimizu H. |
| タイトル | Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms. |
| 巻・号 | 78(1) |
| ページ | 151-9 |
| 公開日 | 2014-1-1 |
| DOI | 10.1080/09168451.2014.877816 |
| PMID | 25036498 |
| MeSH | Metabolic Engineering / methods* Metabolomics* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics* Succinic Acid / metabolism* |
| IF | 1.516 |
| 引用数 | 25 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY6313(BY4739) FYG16_m23 BYP803(pNV11) |