RRC ID 32215
Author Shiba K, Inaba K.
Title Distinct roles of soluble and transmembrane adenylyl cyclases in the regulation of flagellar motility in Ciona sperm.
Journal Int J Mol Sci
Abstract Adenylyl cyclase (AC) is a key enzyme that synthesizes cyclic AMP (cAMP) at the onset of the signaling pathway to activate sperm motility. Here, we showed that both transmembrane AC (tmAC) and soluble AC (sAC) are distinctly involved in the regulation of sperm motility in the ascidian Ciona intestinalis. A tmAC inhibitor blocked both cAMP synthesis and the activation of sperm motility induced by the egg factor sperm activating and attracting factor (SAAF), as well as those induced by theophylline, an inhibitor of phoshodiesterase. It also significantly inhibited cAMP-dependent phosphorylation of a set of proteins at motility activation. On the other hand, a sAC inhibitor does not affect on SAAF-induced transient increase of cAMP, motility activation or protein phosphorylation, but it reduced swimming velocity to half in theophylline-induced sperm. A sAC inhibitor KH-7 induced circular swimming trajectory with smaller diameter and significantly suppressed chemotaxis of sperm to SAAF. These results suggest that tmAC is involved in the basic mechanism for motility activation through cAMP-dependent protein phosphorylation, whereas sAC plays distinct roles in increase of flagellar beat frequency and in the Ca2+-dependent chemotactic movement of sperm.
Volume 15(8)
Pages 13192-208
Published 2014-7-28
DOI 10.3390/ijms150813192
PII ijms150813192
PMID 25073090
PMC PMC4159788
MeSH Adenylyl Cyclase Inhibitors Adenylyl Cyclases / classification Adenylyl Cyclases / metabolism* Animals Bicarbonates / pharmacology Calcium / metabolism Ciona intestinalis / enzymology Ciona intestinalis / metabolism Cyclic AMP / metabolism Enzyme Inhibitors / pharmacology Male Phosphorylation / drug effects Phylogeny Sperm Motility / drug effects Spermatozoa / enzymology Spermatozoa / physiology* Testis / enzymology Theophylline / pharmacology Valinomycin / pharmacology
IF 3.687
Times Cited 7
C.intestinalis / (O.japonicus) Wild C. int