| RRC ID |
32238
|
| Author |
Mochida K, Hasegawa A, Otaka N, Hama D, Furuya T, Yamaguchi M, Ichikawa E, Ijuin M, Taguma K, Hashimoto M, Takashima R, Kadota M, Hiraiwa N, Mekada K, Yoshiki A, Ogura A.
|
| Title |
Devising assisted reproductive technologies for wild-derived strains of mice: 37 strains from five subspecies of Mus musculus.
|
| Journal |
PLoS One
|
| Abstract |
Wild-derived mice have long offered invaluable experimental models for mouse genetics because of their high evolutionary divergence from laboratory mice. A number of wild-derived strains are available from the RIKEN BioResource Center (BRC), but they have been maintained as living stocks because of the unavailability of assisted reproductive technology (ART). In this study, we sought to devise ART for 37 wild-derived strains from five subspecies of Mus musculus maintained at the BRC. Superovulation of females was effective (more than 15 oocytes per female) for 34 out of 37 strains by treatment with either equine chorionic gonadotropin or anti-inhibin serum, depending on their genetic background (subspecies). The collected oocytes could be fertilized in vitro at mean rates of 79.0% and 54.6% by the optimized protocol using fresh or frozen-thawed spermatozoa, respectively. They were cryopreserved at the 2-cell stage by vitrification with an ethylene glycol-based solution. In total, 94.6% of cryopreserved embryos survived the vitrification procedure and restored their normal morphology after warming. A conventional embryo transfer protocol could be applied to 25 out of the 35 strains tested. In the remaining 10 strains, live offspring could be obtained by a modified embryo transfer protocol using cyclosporin A treatment and co-transfer of ICR (laboratory mouse strain) embryos. Thus, ART for 37 wild-derived strains was devised successfully and is now routinely used for their preservation and transportation. The information provided here might facilitate broader use and wider distribution of wild-derived mice for biomedical research.
|
| Volume |
9(12)
|
| Pages |
e114305
|
| Published |
2014-1-1
|
| DOI |
10.1371/journal.pone.0114305
|
| PII |
PONE-D-14-44523
|
| PMID |
25470728
|
| PMC |
PMC4254977
|
| MeSH |
Animal Husbandry
Animals
Breeding / methods*
Cryopreservation*
Embryo Transfer
Female
Male
Mice, 129 Strain
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Oocytes*
Reproductive Techniques, Assisted
Spermatozoa*
|
| IF |
2.74
|
| Times Cited |
12
|
|
WOS Category
|
REPRODUCTIVE BIOLOGY
|
| Resource |
| Mice |
RBRC00207
RBRC00209
RBRC00213
RBRC00265
RBRC00266
RBRC00427
RBRC00428
RBRC00429
RBRC00430
RBRC00431
RBRC00436
RBRC00639
RBRC00653
RBRC00654
RBRC00655
RBRC00657
RBRC00659
RBRC00667
RBRC00738
RBRC00841
RBRC01140
RBRC01164
RBRC01167
RBRC01168
RBRC01196
RBRC01237
RBRC01238
RBRC01241
RBRC01242
RBRC01243
RBRC01245
RBRC01247
RBRC01249
RBRC01341
RBRC01770
RBRC01837
RBRC03108 |
