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RRC ID 32238
著者 Mochida K, Hasegawa A, Otaka N, Hama D, Furuya T, Yamaguchi M, Ichikawa E, Ijuin M, Taguma K, Hashimoto M, Takashima R, Kadota M, Hiraiwa N, Mekada K, Yoshiki A, Ogura A.
タイトル Devising assisted reproductive technologies for wild-derived strains of mice: 37 strains from five subspecies of Mus musculus.
ジャーナル PLoS One
Abstract Wild-derived mice have long offered invaluable experimental models for mouse genetics because of their high evolutionary divergence from laboratory mice. A number of wild-derived strains are available from the RIKEN BioResource Center (BRC), but they have been maintained as living stocks because of the unavailability of assisted reproductive technology (ART). In this study, we sought to devise ART for 37 wild-derived strains from five subspecies of Mus musculus maintained at the BRC. Superovulation of females was effective (more than 15 oocytes per female) for 34 out of 37 strains by treatment with either equine chorionic gonadotropin or anti-inhibin serum, depending on their genetic background (subspecies). The collected oocytes could be fertilized in vitro at mean rates of 79.0% and 54.6% by the optimized protocol using fresh or frozen-thawed spermatozoa, respectively. They were cryopreserved at the 2-cell stage by vitrification with an ethylene glycol-based solution. In total, 94.6% of cryopreserved embryos survived the vitrification procedure and restored their normal morphology after warming. A conventional embryo transfer protocol could be applied to 25 out of the 35 strains tested. In the remaining 10 strains, live offspring could be obtained by a modified embryo transfer protocol using cyclosporin A treatment and co-transfer of ICR (laboratory mouse strain) embryos. Thus, ART for 37 wild-derived strains was devised successfully and is now routinely used for their preservation and transportation. The information provided here might facilitate broader use and wider distribution of wild-derived mice for biomedical research.
巻・号 9(12)
ページ e114305
公開日 2014-1-1
DOI 10.1371/journal.pone.0114305
PII PONE-D-14-44523
PMID 25470728
PMC PMC4254977
MeSH Animal Husbandry Animals Breeding / methods* Cryopreservation* Embryo Transfer Female Male Mice, 129 Strain Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Oocytes* Reproductive Techniques, Assisted Spermatozoa*
IF 2.74
引用数 12
WOS 分野 REPRODUCTIVE BIOLOGY
リソース情報
実験動物マウス RBRC00207 RBRC00209 RBRC00213 RBRC00265 RBRC00266 RBRC00427 RBRC00428 RBRC00429 RBRC00430 RBRC00431 RBRC00436 RBRC00639 RBRC00653 RBRC00654 RBRC00655 RBRC00657 RBRC00659 RBRC00667 RBRC00738 RBRC00841 RBRC01140 RBRC01164 RBRC01167 RBRC01168 RBRC01196 RBRC01237 RBRC01238 RBRC01241 RBRC01242 RBRC01243 RBRC01245 RBRC01247 RBRC01249 RBRC01341 RBRC01770 RBRC01837 RBRC03108