RRC ID 32267
Author Oonuma K, Hirose D, Takatori N, Saiga H.
Title Analysis of the Transcription Regulatory Mechanism of Otx During the Development of the Sensory Vesicle in Ciona intestinalis.
Journal Zool. Sci.
Abstract Establishment of the anterior-posterior axis is an important event in the development of bilateral animals. A homeodomain transcription factor, Otx, is important for the formation of the anterior part of the embryo, and its mRNA is expressed in a continuous manner in a wide range of animals. This pattern of expression is thought to be important for the formation of anterior neural structures, but the mechanism that regulates Otx expression remains largely unknown. Towards understanding how the transcription of Otx is maintained in the cells of anterior neural structure, the sensory vesicle, during embryogenesis, we examined transcription regulatory mechanisms of Otx, using embryos of the ascidian, Ciona intestinalis, from the gastrula to tailbud stages, which have not been studied previously. We identified two genomic regions capable of mimicking the Otx expression pattern from the gastrula to tailbud stages. Putative transcription factor binding sites required for this activity were identified. Notably, distinct sets of transcription factor binding sites were required at different developmental stages for the expression of Otx, suggesting that the continuity of Otx is supported by distinct transcriptional mechanisms in the gastrula and neurula stages. Along with previous studies using Halocynthia roretzi, the present results provide insight into the evolution of transcriptional regulatory mechanism of Otx.
Volume 31(9)
Pages 565-72
Published 2014-9
DOI 10.2108/zs140060
PMID 25186927
MeSH Animals Ciona intestinalis / embryology* Ciona intestinalis / genetics* Embryo, Nonmammalian Gene Expression Regulation, Developmental* Otx Transcription Factors / genetics* Otx Transcription Factors / metabolism* Sensory Receptor Cells / cytology
IF 0.906
Times Cited 0
C.intestinalis / (O.japonicus) Wild C. int