RRC ID 32400
Author Zacharioudaki E, Bray SJ.
Title Tools and methods for studying Notch signaling in Drosophila melanogaster.
Journal Methods
Abstract Notch signaling involves a highly conserved pathway that mediates communication between neighboring cells. Activation of Notch by its ligands, results in the release of the Notch intracellular domain (NICD), which enters the nucleus and regulates transcription. This pathway has been implicated in many developmental decisions and diseases (including cancers) over the past decades. The simplicity of the Notch pathway in Drosophila melanogaster, in combination with the availability of powerful genetics, make this an attractive model for studying fundamental principles of Notch regulation and function. In this article we present some of the established and emerging tools that are available to monitor and manipulate the Notch pathway in Drosophila and discuss their strengths and weaknesses.
Volume 68(1)
Pages 173-82
Published 2014-6-15
DOI 10.1016/j.ymeth.2014.03.029
PII S1046-2023(14)00131-5
PMID 24704358
PMC PMC4059942
MeSH Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Ligands Molecular Biology / methods Receptors, Notch / genetics* Receptors, Notch / metabolism Signal Transduction*
IF 3.998
Times Cited 5
WOS Category BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Drosophila DGRC#107388 DGRC#101292 DGRC#108838 DGRC#108332 DGRC#108330 DGRC#108439