RRC ID 32402
Author Abreu-Blanco MT, Verboon JM, Liu R, Watts JJ, Parkhurst SM.
Title Drosophila embryos close epithelial wounds using a combination of cellular protrusions and an actomyosin purse string.
Journal J Cell Sci
Abstract The repair of injured tissue must occur rapidly to prevent microbial invasion and maintain tissue integrity. Epithelial tissues in particular, which serve as a barrier against the external environment, must repair efficiently in order to restore their primary function. Here we analyze the effect of different parameters on the epithelial wound repair process in the late stage Drosophila embryo using in vivo wound assays, expression of cytoskeleton and membrane markers, and mutant analysis. We define four distinct phases in the repair process, expansion, coalescence, contraction and closure, and describe the molecular dynamics of each phase. Specifically, we find that myosin, E-cadherin, Echinoid, the plasma membrane, microtubules and the Cdc42 small GTPase respond dynamically during wound repair. We demonstrate that perturbations of each of these components result in specific impairments to the wound healing process. Our results show that embryonic epithelial wound repair is mediated by two simultaneously acting mechanisms: crawling driven by cellular protrusions and actomyosin ring contraction along the leading edge of the wound.
Volume 125(Pt 24)
Pages 5984-97
Published 2012-12-15
DOI 10.1242/jcs.109066
PII jcs.109066
PMID 23038780
PMC PMC3585516
MeSH Actomyosin / metabolism* Animals Drosophila / cytology Drosophila / embryology Drosophila / metabolism* Epithelial Cells / cytology Epithelial Cells / metabolism
IF 4.573
Times Cited 75
WOS Category CELL BIOLOGY
Resource
Drosophila DGRC#109007