Reference - Detail
| RRC ID | 32432 |
|---|---|
| Author | Joshi SS, Cheong H, Meller VH. |
| Title | A strategy for generation and balancing of autosome: Y chromosome translocations. |
| Journal | Fly (Austin) |
| Abstract |
We describe a method for generation and maintenance of translocations that move large autosomal segments onto the Y chromosome. Using this strategy we produced ( 2;Y) translocations that relocate between 1.5 and 4.8 Mb of the 2nd chromosome.. All translocations were easily balanced over a male-specific lethal 1 (msl-1) mutant chromosome. Both halves of the translocation carry visible markers, as well as P-element ends that enable molecular confirmation. Halves of these translocations can be separated to produce offspring with duplications and with lethal second chromosome deficiencies . Such large deficiencies are otherwise tedious to generate and maintain. |
| Volume | 8(1) |
| Pages | 58-62 |
| Published | 2014-1-16 |
| DOI | 10.4161/fly.27814 |
| PII | 27814 |
| PMID | 24646827 |
| PMC | PMC3974896 |
| MeSH | Animals Chromosomes, Insect* Drosophila / genetics* Female Genetic Techniques* Male Translocation, Genetic* Y Chromosome* |
| IF | 1.204 |
| Times Cited | 1 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Drosophila | DGRC#125639 DGRC#124255 DGRC#124291 DGRC#124216 DGRC#123609 |