RRC ID 32461
著者 Tchankouo-Nguetcheu S, Udinotti M, Durand M, Meng TC, Taouis M, Rabinow L.
タイトル Negative regulation of MAP kinase signaling in Drosophila by Ptp61F/PTP1B.
ジャーナル Mol Genet Genomics
Abstract PTP1B is an important negative regulator of insulin and other signaling pathways in mammals. However, the role of PTP1B in the regulation of RAS-MAPK signaling remains open to deliberation, due to conflicting evidence from different experimental systems. The Drosophila orthologue of mammalian PTP1B, PTP61F, has until recently remained largely uncharacterized. To establish the potential role of PTP61F in the regulation of signaling pathways in Drosophila and particularly to help resolve its fundamental function in RAS-MAPK signaling, we generated a new allele of Ptp61F as well as employed both RNA interference and overexpression alleles. Our results validate recent data showing that the activity of insulin and Abl kinase signaling is increased in Ptp61F mutants and RNA interference lines. Importantly, we establish negative regulation of the RAS/MAPK pathway by Ptp61F activity in whole animals. Of particular interest, our results document the modulation of hyperactive MAP kinase activity by Ptp61F alleles, showing that the phosphatase intervenes to directly or indirectly regulate MAP kinase itself.
巻・号 289(5)
ページ 795-806
公開日 2014-10-1
DOI 10.1007/s00438-014-0852-2
PMID 24752400
MeSH Animals Compound Eye, Arthropod / enzymology Compound Eye, Arthropod / growth & development Drosophila Proteins / physiology* Drosophila melanogaster / enzymology* Drosophila melanogaster / growth & development Epistasis, Genetic ErbB Receptors / metabolism Female Genetic Association Studies MAP Kinase Signaling System* Male Molecular Sequence Data Protein Tyrosine Phosphatase, Non-Receptor Type 1 / physiology* Protein Tyrosine Phosphatases, Non-Receptor / physiology* Wings, Animal / enzymology Wings, Animal / growth & development
IF 2.797
引用数 4
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
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